- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C11H12N2O6
Molecular Weight: 268.22 g/mol
Exact Mass: 268.07 g/mol
CAS#: 69075-42-9
Purity: ≥ 99 % (HPLC)
Form: solid
Color: white to off-white
Solubility: Up to 200 mM in 1 x PBS or water by heating the obtained suspension for 1 minute by 70 °C, less soluble in DMSO
Spectroscopic Properties: λmax 286 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
RNA synthesis monitoring[1]Description:
Ethynyl-labeled uridine (5-EU) can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo RNA synthesis in proliferating cells. 5-EU is cell permeable and incorporates into nascent RNA instead of its natural analog uridine.The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging[1].
Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Long term storage at < -20°C. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.
-
产品组分
内容
型号
规格 储存温度
5-乙炔基尿苷(5-EU)
CLKN002-01 10mg
-20 °C
5-乙炔基尿苷(5-EU)
CLKN002-02 50mg
-20 °C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
Selected References:
[1] Jao et al. (2008) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Nat. Acad. Sci. USA 105 (41):15779.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.New mRNA poly(A)-tail length is a battleground for coronavirus–host competition
Latifkar Arash, Levdansky Yevgen, ..., Bartel David
bioRxiv | 2025 Oct 10
New Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures.
Michael Hawgood, Bruno Urién, ..., Bennie Lemmens
The EMBO Journal | 2025 Oct 07 | 41057695
A splicing variant in XPA results in delayed onset of clinical features of xeroderma pigmentosum.
Anita van den Heuvel, Annelotte P Wondergem, ..., Martijn S Luijsterburg
The Journal of investigative dermatology | 2025 Sep 24 | 41005614
Composition and RNA binding specificity of metazoan RNase MRP
Yuan Liu, Shiyang He, ..., Jernej Murn
Nucleic Acids Research | 2025 Sep 09
Disruption of nucleolar integrity triggers cellular quiescence through organelle rewiring and secretion
Rawat Prashant, Quaderer Tabea, ..., Peter Matthias
bioRxiv | 2025 Sep 02
The CGG triplet repeat binding protein 1 counteracts R-loop induced transcription-replication stress
Henning Ummethum, Augusto C Murriello, ..., Stephan Hamperl
EMBO Reports | 2025 Aug 26 | 40859011
PAF1C-driven restoration of RNAPII elongation after DNA damage occurs independently of transcription-associated histone mark deposition
van Schie Janne J.M., de Groot Bram A.F.J., Luijsterburg Martijn S.
bioRxiv | 2025 Jul 25
Oncogenic YAP sensitizes cells to CHK1 inhibition via CDK4/6 driven G1 acceleration
Dörthe Gertzmann, Cornelius Presek, ..., Stefan Gaubatz
EMBO Reports | 2025 Jul 04 | 40615577
Mapping and engineering RNA-driven architecture of the multiphase nucleolus.
Sofia A Quinodoz, Lifei Jiang, ..., Clifford P Brangwynne
Nature | 2025 Jul 02 | 40604277
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
