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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped at ambient temperature
Storage Conditions: store at 4°C or -20°C
Shelf Life: 12 months
Form: liquid
Concentration: 5 x
Description:
Direct Extraction Buffer allows an easy and fast extraction of DNA and RNA directly from blood, swabs and animal- or plant tissue. The buffer is optimized for use in combination with Direct PCR or RT-PCR master mixes like qPCR ProbesMaster (PCR-396) or SCRIPT Direct RT-qPCR ProbesMaster (PCR-528).The mix allows DNA and RNA preparation within 3-5 minutes and with a minimum of pipetting steps. It is especially recommended for:
- Direct detection of viral or bacterial DNA in nasal or throat swabs
- Direct PCR from blood samples
- Direct amplification of target DNA from various tissue samples
- Point-of-Care diagnostics
The preparation process can be easily automatized.
Content:
Direct Extraction Buffer
5 x conc.Sample preparation
a) Blood Samples / Liquid Samples- Dilute 5x Extraction Buffer to 1x concentrated Buffer with PCR-grade water.
- Transfer 2 μl of the Blood/Liquid Sample into a tube containing 100 μl to 200 μl of 1x concentrated Extraction Buffer (a dilution of Blood 1:50 to 1:100 in 1x Extraction Buffer is recommended).
- Close the tube and vortex for 15 sec
- Incubate the tube at room temperature (20-25 °C) for 2-3 min.
- Transfer 1-2 μl of the supernatant into a 20 μl qPCR assay or 2-5 μl into a 50 μl qPCR assay.
b) Samples from nasal or throat swabs
- Dilute 5x Extraction Buffer to 1x concentrated Buffer with PCR-grade water.
- Transfer 200 μl 1x Extraction Buffer into a 1.5 ml microtube
- Cut off the cotton tip with the collected nasal or throat swab and place it in the micro tube
- Close the tube and vortex for 15 sec
- Incubate at room temperature (20-25 °C) for 2-3 min
- Remove the cotton tip and squeeze it out at the rim of the tube
- Centrifuge briefly and transfer 1-2 μl of the supernatant into a 20 μl qPCR assay or 2-5 μl into a 50 μl qPCR assay.
c) Samples from Animal or Plant Tissue
- Dilute 5x Extraction Buffer to 1 x concentrated Buffer with PCR-grade water.
- Prepare a small piece from animal or plant tissue not exceeding 8 mm in diameter
- Crack plant seeds to less than 1 mm in diameter using a BeadBeater, Tissue Lyser or small hammer
- Place the sample in a 1.5 ml microtube
- Add 1x concentrated Extraction Buffer to the tissue sample as following:
Sample size (diameter) 1-2 mm 3-4 mm 5-8 mm 1x Extraction Buffer 50 μl 100 μl 200 μl - Mix briefly by tapping or vortexing and make sure that the sample is soaked with Extraction Buffer
- Incubate at room temperature (20-25 °C) for 3 min
- Centrifuge briefly and transfer 1-2 μl of the supernatant into a 20 μl qPCR assay or 2-5 μl into a 50 μl qPCR assay
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产品组分
内容 型号
规格 储存温度
DNA/RNA直接提取缓冲液
PCR534-01 20ml
-20°C DNA/RNA直接提取缓冲液
PCR534-02 100ml
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
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