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番茄凝集素(Tomato lectin,lycopersicon Esculentum Lectin(LEL))，是一种非常稳定的单亚基糖蛋白，约含有等量的蛋白质和碳水化合物。据报道其糖类部分由85%阿拉伯糖和15%半乳糖组成，在溶液中可能形成多聚体聚合物。番茄凝集素虽然和土豆凝集素、曼陀罗凝集素(DSL)和麦胚凝集素(WGA)有部分相同的特异性，但在许多多面呈现出独特之处。番茄凝集素LEL能够很好地与血型糖蛋白(glycophorin)和Tamm-Horsfial糖蛋白结合，能够有效结合和标记啮齿类动物的血管内皮(vascular endothelium)。
实验参考方法
Histochemistry:
1a. Staining procedure for paraffin sections: Deparaffinize and hydrate tissue sections through xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water. If required, retrieve antigens using the Antigen Unmasking Solution.
1b. Staining procedure for frozen sections: Air dry sections. Immediately before staining, fix sections with acetone. Transfer slices to buffer. If endogenous enzyme activities are present, inactivate using appropriate methods.
2. Perform Streptavidin/Biotin blocking if required following kit instructions. Avidin in the Avidin/Biotin Blocking Kit is a glycoprotein containing terminal mannose residues. Use of the Avidin/Biotin Blocking Kit is not recommended especially when using mannose-specific lectins.
3. Block non-specific binding by incubating section with Carbo-Free™ Blocking Solution for 30 minutes at room temperature. Blot excess blocking solution from the sections.
4. Apply biotinylated lectin at approximately 2-20 µg/ml in PBS (10 mM sodium phosphate, 150 mM NaCl, pH 7.4) to the sections and incubate for 30 minutes at room temperature. Wash with TPBS (PBS + 0.05% Tween™ 20).
5. Prepare VECTASTAIN® ® ABC (peroxidase) or VECTASTAIN® ABC-AP (alkaline phosphatase) reagents according to the kit instructions. Apply to the sections and incubate for 30 minutes at room temperature. Wash with TPBS.
6. Apply an appropriate precipitating substrate for the enzyme system used in step 4. For peroxidase, ImmPACT™ DAB is recommended; for alkaline phosphatase, ImmPACT™ Vector® Red. Rinse in tap water.
7. Counterstain (optional), clear and mount. For galactose or GalNAc-specific lectins we recommend that sections be mounted in VECTASHIELD® HardFSet™ Mounting Medium or VECTASHIELD® HardFSet™ Mounting Medium with DAPI.
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产品组分
内容 型号 规格 储存温度
生物素化番茄凝集素
GC26645-01 1mg
-20°C 生物素化番茄凝集素
GC26645-02 2mg
-20°C 操作手册
1 1 常温
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保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
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