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基因组DNA表观遗传学改变的核心机制是CpG岛在特异性基因中的过甲基化以及总体DNA低甲基化。特异性区域DNA甲基化主要发生在启动子中的5’-CpG-3’ 二核苷酸上，或在基因的第一个外显子上，这是抑制疾病细胞中基因转录的一个重要的途径。全基因组DNA低甲基化极有可能是由不同环境影响导致的甲基缺乏引起。已经证明，DNA甲基化改变与各种疾病具有联系，尤其是癌症。高度专一性的甲基化DNA提取可以给正常或患有疾病的细胞提供更具优势、方便快捷以及全面的甲基化状态识别，例如癌细胞，从而推动发展癌症新的诊断和治疗方法。现在已经有多种方法获得浓缩的甲基化DNA，包括甲基—CpG结合域（MBD）基于甲基化DNA亲和柱和甲基化DNA免疫共沉淀反应。然而迄今为止，这些方法都相当的耗费时间，耗费劳力，低产出，更糟的是，还需要纯化的DNA作为起始材料。我司组织DNA甲基化免疫共沉淀反应试剂盒（TMeDIP）拥有其专有独特的步骤/构成，从各种哺乳动物组织中提取甲基化DNA。在实验中，甲基胞嘧啶特异性抗体被使用来做全基因组DNA甲基化免疫共沉淀反应。然后，免疫共沉淀甲基化的片段可以用来做标准DNA检测。
高度特异的DNA甲基化的富集应该提供一个优势且方便，可以综合识别的甲基化状态的正常和病变细胞，例如癌细胞，这可能导致开发新的诊断和治疗癌症的方法。几个方法已用于富集甲基化DNA，包括甲基CpG结合域(MBD)，来建立甲基化DNA亲和柱和免疫沉淀反应。然而，到目前为止这些方法相当耗时,耗力、低产量，尤其是，需要净化DNA为原料。下面是胞嘧啶的甲基化、羟甲基化形式：
开发的组织MeDIP试剂盒。是专门针对各种组织样本而设计的，具有如下的优势和特性： 直接对组织裂解物DNA甲基化片段做免疫共沉淀 高效获得浓缩甲基化DNA> 95%。
最快的操作步骤，仅需3.5小时。
联管孔板设计使实验更灵活：手动，高产出。
DNA纯化柱设计：省时省力。
兼容后续所有采用不同方法的PCR扩增实验
简单，可信，实验条件始终如一。
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型号：A-P-2020-24(24次)
型号：A-P-2020-48(48次)
储存温度
MC1
抗体缓冲液
8 ml 16 ml 4°CMC2 反应缓冲液 4 ml 8 ml 室温 MC3 清洗缓冲液 16 ml 32 ml 4°C MC4 DNA 释放液 2 ml 4 ml 室温 CP5 结合液 5 ml 8 ml 室温 CP6 洗脱液 0.6 ml 1.2 ml 室温 NMI Normal-Mouse IgG(1mg/ml)* 10 ul 20 ul 4°C 蛋白酶K（10mg/ml）* 25 ul 50 ul 4°C 甲基化抗体K(1mg/ml)* 25 ul 50 ul 4°C 带裙边条状8联管 3条 6条 4°C 粘性封口膜 3片 6片 室温 FC F-spin Column* 30个 50个 室温 FCT
F-Collection Tube
30个 50个 室温 操作手册
1
1 室温
*注意：在将离心柱放入微型离心机之前，始终将离心柱盖子旋紧盖严。
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FAQ
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Signoretti C et. al. (March 2023). Studies in CRISPR-generated Mediterranean G6PD variant rats reveal G6PD orchestrates genome-wide DNA methylation and gene expression in vascular wall. bioRxiv.
Yuan X et. al. (January 2023). Genome-wide DNA methylation analysis of discordant monozygotic twins reveals consistent sites of differential methylation associated with congenital heart disease. Genomics. 115(2):110565.
Jacob C et. al. (November 2022). Mediterranean G6PD variant mitigates expression of DNA methyltransferases and right heart pressure in experimental model of pulmonary hypertension. J Biol Chem. 298(12):102691.
Lee HG et. al. (April 2019). Tipping points of gastric pH regulation and energetics in the sea urchin larva exposed to CO<sub>2</sub> induced seawater acidification. Comp Biochem Physiol A Mol Integr Physiol.
Li GL et. al. (January 2017). MeDIP-seq reveals the features of mitochondrial genomic methylation in immature testis of Chinese mitten crab Eriocheir sinensis. Mitochondrial DNA A DNA Mapp Seq Anal. :1-8.
Wu GC et. al. (April 2016). The Testis Is a Primary Factor That Contributes to Epigenetic Modifications in the Ovaries of the Protandrous Black Porgy, Acanthopagrus schlegelii. Biol Reprod.
Hong S et. al. (January 2015). Epigenetic regulation of genes that modulate chronic stress-induced visceral pain in the peripheral nervous system. Gastroenterology. 148(1):148-157.e7.
Tyagi E et. al. (January 2015). Interactive actions of Bdnf methylation and cell metabolism for building neural resilience under the influence of diet. Neurobiol Dis. 73:307-18.
Anier K et. al. (March 2014). Maternal separation is associated with DNA methylation and behavioural changes in adult rats. Eur Neuropsychopharmacol. 24(3):459-68.
Anier K et. al. (October 2013). S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice. Int J Neuropsychopharmacol. 16(9):2053-66.
Calabrese F et. al. (August 2013). Lack of serotonin transporter alters BDNF expression in the rat brain during early postnatal development. Mol Neurobiol. 48(1):244-56.
Zhang X et. al. (January 2013). Promoter hypermethylation of ARID1A gene is responsible for its low mRNA expression in many invasive breast cancers. PLoS One. 8(1):e53931.
Anier K et. al. (November 2010). DNA methylation regulates cocaine-induced behavioral sensitization in mice. Neuropsychopharmacology. 35(12):2450-61.
Aoyama T et. al. (September 2010). Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells. J Biol Chem. 285(39):29842-50.
Bechtel W et. al. (May 2010). Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 16(5):544-50.
Molteni R et. al. (March 2010). Reduced function of the serotonin transporter is associated with decreased expression of BDNF in rodents as well as in humans. Neurobiol Dis. 37(3):747-55.
Roth TL et. al. (May 2009). Lasting epigenetic influence of early-life adversity on the BDNF gene. Biol Psychiatry. 65(9):760-9.
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