雌二醇荧光剂

雌二醇荧光剂，Estradiol Glow，是一种17-ß-雌二醇，采用新型方法标记了低分子量生物相容性荧光团，保留了这种卵巢激素的生物活性。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

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雌二醇荧光剂

所属分类

染料探针

目录号

PR958

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迄今为止，对靶组织中类固醇敏感细胞的鉴定尝试采用了放射性标记激素结合放射自显影技术，或受体蛋白的原位杂交与免疫组织化学技术。已有大量研究使用经酶、荧光团或免疫原性标记物标记的类固醇激素，但这些标记都会导致激素的生物学特性和功能丧失。雌二醇荧光剂是一种17-ß-雌二醇，采用新型方法标记了低分子量生物相容性荧光团，因此保留了这种卵巢激素的生物活性。对实验动物进行全身注射后，发现该荧光类固醇在已知的雌激素靶组织（包括大脑）的细胞核中积累。

雌二醇荧光技术为细胞摄取、细胞内转运和亚细胞结合提供了新见解。快速类固醇作用的机制在单细胞层面变得清晰可见。

For general laboratory use.

Shipping: shipped at 4 °C

Storage Conditions: store at 4 °C
store dark

Shelf Life: 12 months

Molecular Formula: C₄₇H₆₇BrN₂O₂

Molecular Weight: 771.95 g/mol

Purity: > 97 % (DC, NMR)

Form: lyophilised, glassy to waxy appearance

Color: brownish-red

Solubility: High: MeOH, EtOH, PrOH, DMSO, DMF Moderate: acetone, CH₂Cl₂, CH₃Cl, THF Low: H₂O and aqueous buffer systems (after presolution in DMSO >1 μM) Insoluble: Et₂O, hydrocarbons such as hexane, heptane, benzene

Spectroscopic Properties: λ_exc 467 nm, λ_em 618 nm, ε 29.0 L mmol^-1 cm^-1 (PBS pH 7.4) λ_exc 501 nm, λ_em 596 nm, ε 52.0 L mmol^-1 cm^-1 (ethanol)

Description:
Estradiol Glow is the ovarian steroid hormone 17-ß-Estradiol labeled with a novel low molecular weight orange/red fluorophor thus retaining its chemical properties and it´s biological activity. This allows for a wide range of applications including
- analysis of in vivo and in vitro steroid uptake in real time
- monitoring of intracellular and subcellular steroid transport
- studies on steroid-binding proteins
- use as tracer for steroid immunoassays (to replace isotopes or enzymes
- photodynamic elimination of estrogen sensitive tissues and cells in experimental settings with possible future clinical implications
Estradiol Glow can be histologically fixed using formaldehyde or glutaraldehyde without any significant loss of fluorescence intensity.
Typical concentration range used for cell culture experiments: 10^-5M - 10^-7M (may vary depending on cell line and individual experimental set-up).
关键词:
- 雌二醇荧光剂

产品组分

内容	型号	规格	储存温度
雌二醇荧光剂	PR958-01	100ug	-20°C
雌二醇荧光剂	PR958-02	500ug	-20°C
操作手册	1	1	常温

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Selected References:
Jirikowski et al. (2011) Uptake, intracellular transport and physiological effects of biologically active fluorescent steroids. Rapid Responses to Steroid Hormones. 7th International Meeting, 14-17 September 2011, Crete, Greece.