氨基烯丙基-dUTP-二乙基氨基香豆素
氨基烯丙基-dUTP-二乙基氨基香豆素，DEAC-dUTP，Diethylaminocoumarin-5-dUTP，5-二乙氨基香豆素脱氧尿苷三磷酸，Diethylaminocoumarin-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt，二乙氨基香豆素-(5-氨基烯丙基)-2'-脱氧尿苷-5'-三磷酸，三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
关键词
氨基烯丙基-dUTP-二乙基氨基香豆素
所属分类
目录号
NU803DEAC
- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C26H33N4O17P3 (free acid)
Molecular Weight: 766.48 g/mol (free acid)
Exact Mass: 766.11 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in 10 mM Tris-HCl
Color: yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 426 nm, λem 480 nm, ε 57.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I in-house dataDescription:
DEAC-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.Recommended DEAC-dUTP/dTTP ratio for Nick Translation: 30-50% DEAC-dUTP/ 70-50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
-
产品组分
内容
型号
规格 储存温度
氨基烯丙基-dUTP-二乙基氨基香豆素
NU803DEAC-01 25ul（1mM）
-20°C 氨基烯丙基-dUTP-二乙基氨基香豆素
NU803DEAC-02 50ul（1mM）
-20°C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
The Precise Breakpoint Mapping in Paracentric Inversion 10q22.2q23.3 by Comprehensive Cytogenomic Analysis, Multicolor Banding, and Single-Copy Chromosome Sequencing.
Tatyana V Karamysheva, Tatyana A Gayner, ..., Nikolay B Rubtsov
Biomedicines | 2022 Dec 14 | 36552011
Assessing the Potential of Using the Langdon 5D(5B) Substitution Line for the Introgression of Aegilops tauschii Into Durum Wheat
Manel Othmeni, Surbhi Grewal, ..., Julie King
Frontiers in Plant Science | 2022 Jul 07 | 35873983
Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers
Julie King, Surbhi Grewal, ..., Ian Phillip King
Frontiers in Plant Science | 2022 Jun 01 | 35720607
Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers
King Julie, Grewal Surbhi, ..., King Ian
bioRxiv | 2022 May 05
Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines.
Surbhi Grewal, Benedict Coombes, ..., Julie King
The plant genome | 2022 Apr 20 | 35102721
A novel approach to develop wheat chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in doubled haploid introgression lines
Grewal Surbhi, Coombes Benedict, ..., King Julie
bioRxiv | 2021 Oct 01
Generation of Doubled Haploid Wheat- Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers
Surbhi Grewal, Veronica Guwela, ..., Julie King
Frontiers in Plant Science | 2021 May 13 | 34054892
Development of Wheat- Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
Surbhi Grewal, Manel Othmeni, ..., Julie King
Frontiers in Plant Science | 2020 May 14 | 32477394
Development of Wheat- Aegilops caudata Introgression Lines and Their Characterisation Using Genome-Specific KASP Markers
Grewal Surbhi, Othmeni Manel, ..., King Julie
bioRxiv | 2020 Mar 04
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
