氨基烯丙基-dUTP-ATTO-425
氨基烯丙基-dUTP-ATTO-425，Aminoallyl-dUTP-ATTO-425，5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 425, Triethylammonium salt，5-（3-氨基烯丙基）-2'-脱氧尿苷-5'-三磷酸，用ATTO 425标记，三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
关键词
氨基烯丙基-dUTP-ATTO-425
所属分类
目录号
NU803425
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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C34H45N4O19P3 (free acid)
Molecular Weight: 906.66 g/mol (free acid)
Exact Mass: 906.16 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: green-yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 439 nm, λem 485 nm, ε 45.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house dataDescription:
Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.Recommended Aminoallyl-dUTP-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-ATTO425/ 80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-ATTO425/ 70-50% dTTP (Nick Translation)
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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产品组分
内容
型号
规格 储存温度
氨基烯丙基-dUTP-ATTO-425
NU803425-01 10ul（1mM）
-20°C 氨基烯丙基-dUTP-ATTO-425
NU803425-02 50ul（1mM）
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
Basinko et al. (2012) Clinical and molecular cytogenetic studies in ring chromosome 5: report of a child with congenital abnormalities.Eur. J. Med. Genet. 55 (2):112.Growing functional artificial cytoskeletons in the viscoelastic confinement of DNA synthetic cells
Nature Chemical Engineering | 2025 Oct 07
Satellite DNAs and the evolution of the multiple X 1 X 2 Y sex chromosomes in the wolf fish Hoplias malabaricus (Teleostei; Characiformes)
Gustavo Akira Toma, Alexandr Sember, ..., Marcelo de Bello Cioffi
Scientific reports | 2024 Sep 02 | 39223262
Enzymatic Synthesis of Aptamer-Polynucleotide Nanoparticles with High Anticancer Drug Loading for Targeted Delivery.
Sonal Deshpande, Yunqi Yang, ..., Ashutosh Chilkoti
Biomacromolecules | 2024 Jan 09 | 38051194
Highly Rearranged Karyotypes and Multiple Sex Chromosome Systems in Armored Catfishes from the Genus Harttia (Teleostei, Siluriformes)
Geize Aparecida Deon, Larissa Glugoski, ..., Orlando Moreira-Filho
Genes | 2020 Nov 18 | 33218104
Multiple Sex Chromosomes and Evolutionary Relationships in Amazonian Catfishes: The Outstanding Model of the Genus Harttia (Siluriformes: Loricariidae)
Francisco de M. C. Sassi, Geize A. Deon, ..., Marcelo B. Cioffi
Genes | 2020 Oct 10 | 33050411
The Malaria-Protective Human Glycophorin Structural Variant DUP4 Shows Somatic Mosaicism and Association with Hemoglobin Levels
Walid Algady, Sandra Louzada, ..., Edward J. Hollox
American Journal of Human Genetics | 2018 Nov 01 | 30388403
Patent Method, probe and kit for DNA in situ hybridization and use thereof
Laurent Arnaud Chatre, Miria Ricchetti
us09957555 | 2018 May 01
Patent CRISPR oligonucleotides and gene editing
Namritha Ravinder, Korbinian Heil, ..., Sanjay Kumar
us09879283 | 2018 Jan 30
Large heterogeneity of mitochondrial DNA transcription and initiation of replication exposed by single-cell imaging.
Laurent Chatre, Miria Ricchetti
Journal of cell science | 2013 Nov 19 | 23239030
Prevalent coordination of mitochondrial DNA transcription and initiation of replication with the cell cycle.
Laurent Chatre, Miria Ricchetti
Nucleic acids research | 2013 May 02 | 23345615
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