氨基烯丙基-dUTP-XX-AZDye488
氨基烯丙基-dUTP-XX-AZDye488，Aminoallyl-dUTP-XX-AZDye488，5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with AZDye 488, Triethylammonium salt，5-（3-氨基烯丙基）-2'-脱氧尿苷-5'-三磷酸，标记有AZDye 488，三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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氨基烯丙基-dUTP-XX-AZDye488
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目录号
NU803XXAZ488
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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C45H54N7O26P3S2 (free acid)
Molecular Weight: 1265.99 g/mol (free acid)
Exact Mass: 1265.18 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in 10 mM Tris-HCl
Color: yellow to orange
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 494 nm, λem 515 nm, ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house dataDescription:
Aminoallyl-dUTP-XX-AZDye488 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AZDye 488 (structural analog to Alexa Fluor 488®) is a hydrophilic dye with excellent photostability compared to fluorescein.Recommended Aminoallyl-dUTP-XX-AZDye488/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-AZDye488/ 70-50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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规格 储存温度
氨基烯丙基-dUTP-XX-AZDye488
NU803XXAZ488-01 10ul（1mM）
-20°C 氨基烯丙基-dUTP-XX-AZDye488
NU803XXAZ488-02 50ul（1mM）
-20°C 操作手册
1 1 常温
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NU803XXAZ488-氨基烯丙基-dUTP-XX-AZDye488
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