氨基烯丙基-dUTP-XX-ATTO-488
氨基烯丙基-dUTP-XX-ATTO-488,Aminoallyl-dUTP-XX-ATTO-488,5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 488, Triethylammonium salt,5-(3-氨基烯丙基)-2'-脱氧尿苷-5'-三磷酸,标记有ATTO 488,三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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氨基烯丙基-dUTP-XX-ATTO-488
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目录号
NU803XX488
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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C49H63N8O25P3S2 (free acid)
Molecular Weight: 1321.11 g/mol (free acid)
Exact Mass: 1320.26 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in 10 mM Tris-HCl
Color: yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 500 nm, λem 520 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house dataDescription:
Aminoallyl-dUTP-XX-ATTO488 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.Recommended Aminoallyl-dUTP-XX-ATTO488/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-ATTO488/ 70-50% dTTP
Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
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氨基烯丙基-dUTP-XX-ATTO-488
NU803XX488-01 10ul(1mM)
-20°C 氨基烯丙基-dUTP-XX-ATTO-488
NU803XX488-02 50ul(1mM)
-20°C 操作手册
1 1 常温
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