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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C20H28N10O19P4 (free acid)
Molecular Weight: 836.39 g/mol (free acid)
Exact Mass: 836.05 g/mol (free acid)
CAS#: 5542-28-9
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
关键词:- AP4A(AppppA)溶液
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产品组分
内容
型号
规格 储存温度
AP4A(AppppA)溶液
NU507-01 100ul(10mM)
-20°C AP4A(AppppA)溶液
NU507-02 500ul(10mM)
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ

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Selected References:
Reiser et al. (1999) Nucleotide radiolabels as tools for studying P2Y receptors in membranes from brain and lung tissue.Prog. Brain Res. 129:45.
Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster. Int. J. Biochem. Cell Biol. 39 (5):943.
Gross et al. (2006) Nucleotide-binding domains of Cystic Fibrosis Transmembrane Conductance Regulator, an ABC Transporter, Catalyze Adenylate Kinase Activity but not ATP Hydrolysis. J. Biol. Chem. 281 (7):4058.
Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.
Campbell et al. (1999) Characterization of P1,P4-diadenosine 5'-tetraphosphate binding on bovine aortic endothelial cells. Arch. Biochem. Biophys. 364:280.
Vartanian et al. (1999) Ap4A induces apoptosis in human cultured cells. FEBS Lett. 456:175.
Brevet et al. (1985) Variation of Ap4A and other dinucleoside polyphosphates in stressed Drosophila cells. J. Biol. Chem. 260:15566.
Guedon et al. (1985) Effect of diadenosine tetraphosphate microinjection on heat shock protein synthesis in Xenopus laevis oocytes. EMBO J. 4:3743.
Guranowski et al. (1985) Phosphorolytic cleavage of diadenosine 5',5'''-P1,P4-tetraphosphate. Properties of homogeneous diadenosine 5',5'''-P1,P4-tetraphosphate alpha, beta-phosphorylase from Saccharomyces cerevisiae. J. Biol. Chem. 260:3542.
Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.
Guranowski et al. (1983) Catabolism of diadenosine 5',5'-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5',5'-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12. J. Biol. Chem. 258:14784.
Jakubowski et al. (1983) Enzymes hydrolyzing ApppA and/or AppppA in higher plants. Purification and some properties of diadenosine triphosphatase, diadenosine tetraphosphatase, and phosphodiesterase from yellow lupin (Lupinus luteus) seeds. J. Biol. Chem. 258:9982.
Giammarinaro et al. (2022) Diadenosine tetraphosphate regulates biosynthesis of GTP in Bacillus subtilis. Nat Microbiol 7:1442
Young et al. (2024) From dusty shelves toward the spotlight: growing evidence for Ap4A as an alarmone in maintaining RNA stability and proteostasis. Current Opinion in Microbiology 81:102536.
Zegarra et al. (2023) The mysterious diadenosine tetraphosphate (AP4A). microLife 4: doi.org/10.1093/femsml/uqad016.Systematic P2Y receptor survey identifies P2Y11 as modulator of immune responses and virus replication in macrophages.
Line Lykke Andersen, Yiqi Huang, ..., Andreas Pichlmair
The EMBO journal | 2023 Dec 05 | 37881155
Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes.
Kathrin H Götz, Martin Mex, ..., Andreas Marx
Cell chemical biology | 2020 Aug 27 | 31492597
Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes.
Kathrin H Götz, Martin Mex, ..., Andreas Marx
Cell chemical biology | 2020 Aug 27 | 31492597
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