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本制品和传统的T4连接酶原理不同，它利用了Topoisomerase可以在瞬间（几秒钟-几分钟）、高效（接近100%）连接DNA片段的原理采用本公司独创的工艺制成。
1. 平末端PCR产物不需要先加A，在瞬间（几秒钟-几分钟）完成平末端PCR产物连接。
2. 特制的新型载体质粒大小仅仅不到2kb，充分发挥了TOPO载体越小，可容纳片段越大的优势，最大限度提高了大片段连接效率；连接后质粒大小比传统载体小2kb以上，质粒越小，转化效率越高，极大的提高了各种片段连接后的转化子数量。
3. 采用氨苄抗性载体只需0-10分钟复苏时间，比卡那抗性载体1小时复苏时间缩短6倍。
4. 最快可以省略1小时复苏步骤，热休克冰浴后，不用加LB复苏，直接将感受态涂板即可。从连接到涂板全程只需15分钟。
5. 自杀基因零背景原理，无自连假阳性，无需繁琐蓝白斑筛选和菌落PCR筛选。大部分情况下随机挑一个克隆便是有插入的（接近100%）。
6. 连接长片段能力远超传统Blunt克隆载体，可连接长达10kb片段（例如连接5kb片段，也可能达到挑10个菌落，至少8个是有插入的效果），是新一代世界领先的简单、快速、零背景免筛选的TOPO Blunt平末端克隆载体。
注：本TOPO载体连接体系按照10 μl一次计算可以用20次，如果按照其它公司产品5 μl一次计算，使用次数可以增加一倍，20次实际可用40次。 -
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零背景pTOPO-Blunt平末端克隆试剂盒
CV1601-1 20次x10ul 2-8°C 零背景pTOPO-Blunt平末端克隆试剂盒
CV1601-2 80次x10ul 2-8°C 操作手册
1 1 常温
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保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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1. Development and Application of a Quantitative RT-PCR Approach for Quantification of Grapevine fanleaf virus. Acta Horticulturae Sinica 2016, 43 (3): 538-548.
2. An adenine nucleotide translocase (ANT) gene from Apostichopus japonicus; molecular cloning and expression analysis in response to lipopolysaccharide (LPS) challenge and thermal stress. Fish & Shellfish Immunology 2016, 49: 16–23
3. Cloning and ExpressionAnalysis of AcLOX1 Gene in Onion (Allium cepa). Journal of Agricultural Biotechnology 2016, 24(4): 548-556
4. Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China. Scientific Reports 2016, 6:36406, DOI: 10.1038/srep36406
5. Occurrence and Genetic Diversity of Grapevine berry inner necrosis virus from Grapevines in China. Plant Disease 2017, 101(1): 144-149
6. The complete mitochondrial genome of Euproctis similis (Lepidoptera: Noctuoidea: Erebidae) and phylogenetic analysis. International Journal of Biological Macromolecules 2017, 105: 219-227
7. Isolation and Characterization of Bacillus Amyloliquefaciens L-1 for Biocontrol of Pear Ring Rot. Horticultural Plant Journal 2017, Available online 6 October 2017
8. A new host plant of Cucumber mosaic virus in China-Zinnia elegans. ACTA PHYTOPATHOLOGICA SINICA 2017, 47(3):428-432
9. Analysis of the Complete Genome of Apple stem grooving virus Isolate Jilin-shaguo. Acta Horticulturae Sinica 2018, 45 (4): 641-649.
CV15 Zero Background pTOPO-Blunt Cloning Kit:
1. Detection and genetic diversity analysis of Prunus necrotic ringspot virus in West Liaoning. ACTA PHYTOPATHOLOGICA SINICA 2017, 4Y(1):15-25
2. A 23-Nucleotide Deletion in STK11 Gene Causes Peutz–Jeghers Syndrome and Malignancy in a Chinese Patient Without a Positive Family History. Digestive Diseases and Sciences 2017, 62(11): 3014–3020
3. A novel mutation (c.855delG) in STK11 gene is associated with Peutz–Jeghers syndrome in a Chinese family. Digestive and Liver Disease 2018, 50(3) DOI: 10.1016/j.dld.2017.12.007
CV16 Zero Background pTOPO-Blunt Cloning Kit:
1. Complete nucleotide sequence of a new variant of grapevine fanleaf virus from northeastern China. Arch Virol 2016, doi:10.1007/s00705-016-3101-7
2. Surface Display of Tres Using CotC as a Molecular Vetor on Bacillus subtilis Spores. China Biotechnology 2017, 37(1): 71-80
3. Screening and Identification of Peach Endophytic Bacteria with Antagonism Against Agrobacterium tumefaciens. Scientia Agricultura Sinica 2017, 50(20): 3918-3929
CV17 Zero Background pTOPO-Blunt Simple Cloning Kit::
1. Cloning and Expression Analysis of Citrate Synthase(CS)Gene in Camellia oleifera. Bulletin of Botanical Research 2016, 36(4): 556-564
2. Cloning and expression analysis of a autophagy-related protein 8 encoding gene in Polyporus umbellatus. Chin Med Biotechnol 2016, 11(5): 415-419
3. Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus. Acta Pharmaceutica Sinica B 2017, 7(3): 373–380
4. Development and application of an allele-specific PCR assay for detecting T409C mutation of cyp51 gene linked with tebuconazole resistance in Villosiclava virens (rice false smut) Canadian Journal of Plant Pathology 2017, 39(3)
5. LEA proteins from Gastrodia elata enhance tolerance to low temperature stress in Escherichia coli. Gene 2018, 646: 136-142
6. Complete genomic sequence of a novel macluravirus, alpinia oxyphylla mosaic virus (AloMV), identified in Alpinia oxyphylla. Archives of Virology 2018, https://doi.org/10.1007/s00705-018-3879-62
7. Complete nucleotide sequence of jasmine virus H, a new member of the family Tombusviridae. Archives of Virology 2018, 163(3): 731-735
8. Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species. Archives of Virology 2018, https://doi.org/10.1007/s00705-018-3885-8
9. Endogenous pararetrovirus sequences are widely present in Citrinae genomes. Virus Research 2018, https://doi.org/10.1016/j.virusres.2018.05.018
10. Molecular cloning, polymorphism, and expression analysis of the LKB1/STK11 gene and its association with non-specific digestive disorder in rabbits. Molecular and Cellular 2018, https://doi.org/10.1007/s11010-018-3349-1
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