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本产品包含F8 FastLong聚合酶、dNTPs和优化的反应缓冲液,浓度为2×。使用时只需加入模板、引物,并补足水至Mix终浓度为1×即可。本制品含红色示踪染料,不需添加上样缓冲液即可直接上样进行电泳;也可经过纯化处理,以用于酶切、连接、荧光测序等后续操作。PCR产物3’端带A头,纯化后可直接用IVDSHOW pTOPO TA系列载体(型号:CV14、CV15)克隆。
产品特点:
1. 超快的扩增速度:延伸速度可以达到5-10秒/kb,最短半小时完成PCR。
2. 突出的长片段扩增能力:可以扩增高达10 kb的长片段(包括人类基因组此类的复杂模板)。
3. 良好保真性:保真度是taq的3-6倍以上。
4. 强大扩增能力:产量高,对于一些有特殊结构的复杂模板和GC含量高模板有良好效果。
每一批2 × F8 PCR Mix出货前必须通过下面的2种大小片段的扩增检测:

用50ul反应预混液扩增50ng人基因组DNA片段胶图。M带: 1kb ladder; 1-2带: Hα1AT, 2.6Kb, extension 10 sec/kb; 3-4带: ß-globin, 6kb, extension 15 sec/kb.
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产品组分
内容
型号
规格 储存温度
2×F8 FastLong PCR预混液
PC8001-1 1ml 2-8°C 2×F8 FastLong PCR预混液
PC8001-2 5ml 2-8°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ

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1. Development and Application of a Quantitative RT-PCR Approach for Quantification of Grapevine fanleaf virus. Acta Horticulturae Sinica 2016, 43 (3): 538-548.
2. An adenine nucleotide translocase (ANT) gene from Apostichopus japonicus; molecular cloning and expression analysis in response to lipopolysaccharide (LPS) challenge and thermal stress. Fish & Shellfish Immunology 2016, 49: 16–23
3. Cloning and ExpressionAnalysis of AcLOX1 Gene in Onion (Allium cepa). Journal of Agricultural Biotechnology 2016, 24(4): 548-556
4. Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China. Scientific Reports 2016, 6:36406, DOI: 10.1038/srep36406
5. Occurrence and Genetic Diversity of Grapevine berry inner necrosis virus from Grapevines in China. Plant Disease 2017, 101(1): 144-149
6. The complete mitochondrial genome of Euproctis similis (Lepidoptera: Noctuoidea: Erebidae) and phylogenetic analysis. International Journal of Biological Macromolecules 2017, 105: 219-227
7. Isolation and Characterization of Bacillus Amyloliquefaciens L-1 for Biocontrol of Pear Ring Rot. Horticultural Plant Journal 2017, Available online 6 October 2017
8. A new host plant of Cucumber mosaic virus in China-Zinnia elegans. ACTA PHYTOPATHOLOGICA SINICA 2017, 47(3):428-432
9. Analysis of the Complete Genome of Apple stem grooving virus Isolate Jilin-shaguo. Acta Horticulturae Sinica 2018, 45 (4): 641-649.
CV15 Zero Background pTOPO-Blunt Cloning Kit:
1. Detection and genetic diversity analysis of Prunus necrotic ringspot virus in West Liaoning. ACTA PHYTOPATHOLOGICA SINICA 2017, 4Y(1):15-25
2. A 23-Nucleotide Deletion in STK11 Gene Causes Peutz–Jeghers Syndrome and Malignancy in a Chinese Patient Without a Positive Family History. Digestive Diseases and Sciences 2017, 62(11): 3014–3020
3. A novel mutation (c.855delG) in STK11 gene is associated with Peutz–Jeghers syndrome in a Chinese family. Digestive and Liver Disease 2018, 50(3) DOI: 10.1016/j.dld.2017.12.007
CV16 Zero Background pTOPO-Blunt Cloning Kit:
1. Complete nucleotide sequence of a new variant of grapevine fanleaf virus from northeastern China. Arch Virol 2016, doi:10.1007/s00705-016-3101-7
2. Surface Display of Tres Using CotC as a Molecular Vetor on Bacillus subtilis Spores. China Biotechnology 2017, 37(1): 71-80
3. Screening and Identification of Peach Endophytic Bacteria with Antagonism Against Agrobacterium tumefaciens. Scientia Agricultura Sinica 2017, 50(20): 3918-3929
CV17 Zero Background pTOPO-Blunt Simple Cloning Kit::
1. Cloning and Expression Analysis of Citrate Synthase(CS)Gene in Camellia oleifera. Bulletin of Botanical Research 2016, 36(4): 556-564
2. Cloning and expression analysis of a autophagy-related protein 8 encoding gene in Polyporus umbellatus. Chin Med Biotechnol 2016, 11(5): 415-419
3. Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus. Acta Pharmaceutica Sinica B 2017, 7(3): 373–380
4. Development and application of an allele-specific PCR assay for detecting T409C mutation of cyp51 gene linked with tebuconazole resistance in Villosiclava virens (rice false smut) Canadian Journal of Plant Pathology 2017, 39(3)
5. LEA proteins from Gastrodia elata enhance tolerance to low temperature stress in Escherichia coli. Gene 2018, 646: 136-142
6. Complete genomic sequence of a novel macluravirus, alpinia oxyphylla mosaic virus (AloMV), identified in Alpinia oxyphylla. Archives of Virology 2018, https://doi.org/10.1007/s00705-018-3879-62
7. Complete nucleotide sequence of jasmine virus H, a new member of the family Tombusviridae. Archives of Virology 2018, 163(3): 731-735
8. Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species. Archives of Virology 2018, https://doi.org/10.1007/s00705-018-3885-8
9. Endogenous pararetrovirus sequences are widely present in Citrinae genomes. Virus Research 2018, https://doi.org/10.1016/j.virusres.2018.05.018
10. Molecular cloning, polymorphism, and expression analysis of the LKB1/STK11 gene and its association with non-specific digestive disorder in rabbits. Molecular and Cellular 2018, https://doi.org/10.1007/s11010-018-3349-1
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