ELISTAR Supernova超级ECL发光液

ELISTAR Supernova超级ECL发光液，WESTAR Supernova，超强且较短的信号，可检测低pg的蛋白量，可直接取代市场上同类型产品，无需更改步骤，效果更优。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

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ECL发光液

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K-XLSE2

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内容	型号	规格	储存温度
ELISTAR Supernova超级ECL发光液	K-XLSE2-0010	10ml【100 cm²膜】	4°C
ELISTAR Supernova超级ECL发光液	K-XLSE2-0100	100ml【1000 cm²膜】	4°C
ELISTAR Supernova超级ECL发光液	K-XLSE2-0200	200ml【2000 cm²膜】	4°C
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K-XLSE2-ELISTAR Supernova超级ECL发光液
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Cell Mol Life Sci    |    Mar 18, 2022    |    PubMed ID: 35303162    |    Read Article

Impact of phospholipase C β1 in glioblastoma: a study on the main mechanisms of tumor aggressiveness

Stefano Ratti, Maria Vittoria Marvi, ..., Lucio Cocco

"... (for anti-Mouse) and 1:10’000 (for anti-Rabbit) in PBST for 1 h at room temperature.. Westar Antares and Westar Supernova (Cyanagen, Bologna, Italy) were used as chemiluminescence reagents to detect immunoreactive bands.. Images were captured with the iBright Imaging System (Thermo Fisher Scientific) and samples were ..." (More...)

Biomed Res Int    |    Jan 31, 2022    |    Read Article

Radiosensitizing Effect of Celastrol by Inhibiting G2/M Phase Arrest Induced by the c-myc Gene of Human SW1353 Chondrosarcoma Cells: Network and Experimental Analyses

Jin Qian, Zhen Zhang, ..., Tao Xie

"... Science) in 5% skim milk (1 : 5000) at room temperature for 1 hour.. The protein bands were visualized by using WESTAR SUPERNOVA-1 Kit (Cyanagen) and Molecular Imager (Bio-Rad).. We used ImageJ 1.8.0 software for density analysis." (More...)

Viruses    |    Jan 20, 2022    |    PubMed ID: 35215793    |    Read Article

Heterogeneous Ribonucleoprotein A1 (hnRNPA1) Interacts with the Nucleoprotein of the Influenza a Virus and Impedes Virus Replication

Ramandeep Kaur, Jyoti Batra, ..., Sunil Kumar Lal

"... rabbit (Cell Signaling, Danvers, MA, USA) or IgG mouse antibody (Cell Signaling, USA). ... Chemiluminescent detection of proteins using Immobilon crescendo western blotting HRP substrate detection reagent (Merck Millipore, Burlington, MA, USA) or WESTAR Supernova (Cyanagen, Bologna BO, Italy), using the manufacturer’s protocol.. Proteins bands were captured using the G: Box XX6 imaging system (Syngene, Cambridge, UK) ..." (More...)

Cells    |    Dec 23, 2021    |    PubMed ID: 35011602    |    Read Article

Up-Regulation of Fibroblast Growth Factor 23 Gene Expression in UMR106 Osteoblast-like Cells with Reduced Viability

Sina Münz, Martina Feger, ..., Michael Föller

"... (Ser536; 93H1), anti-GAPDH (D16H11), and anti-rabbit IgG HRP-linked antibody (all from Cell Signaling Technology).. For visualization, membranes were incubated for 2 min with Westar Nova 2.0 (GAPDH) or Westar Supernova (phospho-p65-NFκB) ECL substrate (both from Cyanagen, Bologna, Italy).. The densitometrical analysis was performed on a C-Digit® Blot scanner (Li-Cor, Lincoln, NE, USA) ..." (More...)

Pharmaceutics    |    Dec 20, 2021    |    PubMed ID: 34959480    |    Read Article

Sulfonated Amphiphilic Poly(α)glutamate Amine—A Potential siRNA Nanocarrier for the Treatment of Both Chemo-Sensitive and Chemo-Resistant Glioblastoma Tumors

Adva Krivitsky, Sabina Pozzi, ..., Ronit Satchi-Fainaro

"Secondary horseradish peroxidase (HRP)-conjugated goat anti-rabbit, or goat anti-mouse antibodies (Jackson Immunoresearch, Baltimore, PA, USA; Abcam, Cambridge, UK) were incubated with nitrocellulose membrane at 1:10,000 in TBST for 1 h. Blots were developed using Westar Supernova ECL kit (Cyanagen, Bologna, Italy) in accordance with the manufacturer’s protocol." (More...)

J Cell Mol Med    |    Dec 16, 2021    |    PubMed ID: 34913237    |    Read Article

C1632 suppresses the migration and proliferation of non‐small‐cell lung cancer cells involving LIN28 and FGFR1 pathway, et al. C1632 suppresses the migration and proliferation of non‐small‐cell lung cancer cells involving LIN28 and FGFR1 pathway

Jing-Yi Chen, Yu-Jing Chen, ..., Xiao-Hui Zheng

"... (CST), MMP‐9 (CST), LIN28B (CST), GAPDH (CST), and β‐actin (proteintech) overnight at 4°C, respectively.. Then the lanes were detected with HRP‐conjugated secondary antibodies and visualized using WESTAR SUPERNOVA kit (CYANAGEN)." (More...)

Cell Calcium    |    Nov 1, 2021    |    Read Article

NMDA receptors elicit flux-independent intracellular Ca2+ signals via metabotropic glutamate receptors and flux-dependent nitric oxide release in human brain microvascular endothelial cells

Sharon Negri, Pawan Faris

"Abstract: The excitatory neurotransmitter glutamate gates post-synaptic N-methyl-D-aspartate (NMDA) receptors (NMDARs) to mediate extracellular Ca2+ entry and stimulate neuronal nitric oxide (NO) synthase to release NO and trigger neurovascular coupling (NVC). Neuronal and glial NMDARs may also operate in a flux-independent manner, although it is unclear whether their non-ionotropic mode of action is involved in NVC" (More...)

Int J Mol Sci    |    Oct 23, 2021    |    PubMed ID: 34768882    |    Read Article

The Beneficial Effect of Rosmarinic Acid on Benzophenone-3-Induced Alterations in Human Skin Fibroblasts

Anna Galicka, Joanna Sutkowska-Skolimowska

"... antibody at the concentration of 1:2000 (in TBS-T containing 5% dried milk) was added.. The membranes were incubated for 1 h under gentle shaking, rinsed with TBS-T (5 times for 5 min), and finally subjected to Westar Supernova Chemiluminescent Substrate for Western Blotting (Cyanagen, Bologna, Italy).. The intensity of the protein bands was measured by densitometry using an imaging densitometer ..." (More...)

Int J Mol Sci    |    Sep 10, 2021    |    PubMed ID: 34575952    |    Read Article

Sigma-1 Receptor Agonists Acting on Aquaporin-Mediated H2O2 Permeability: New Tools for Counteracting Oxidative Stress

Giorgia Pellavio, Giacomo Rossino, ..., Umberto Laforenza

"... IgG (Dakocytomation, P0260, Agilent, Cernusco sul Naviglio MI, Italy), diluted 1:120,000 in blocking solution.. The bands were detected with Westar Supernova western blotting detection system (CYANAGEN) and pre-stained molecular weight markers (ab116028, Abcam, Cambridge, UK) used to calculate the molecular weights of the bands.. Blots were stripped following Yeung and Stanley [ ] and reprobed with anti β-2-microglobulin ..." (More...)

Cell Prolif    |    Jul 23, 2021    |    PubMed ID: 34296479    |    Read Article

The different biological effects of TMPyP4 and cisplatin in the inflammatory microenvironment of osteosarcoma are attributed to G‐quadruplex, et al. The different biological effects of TMPyP4 and cisplatin in the inflammatory microenvironment of osteosarcoma are attributed to G‐quadruplex

Jianqiang Chen, Xiangxiang Jin, ..., Guang Liang

"... FAK (CST), p‐FAK (CST), γ‐H2AX (Millipore), RPA (CST) and β‐actin (CST) overnight at 4°C.. The membranes were washed, incubated with the secondary antibody for 1.5 hour and detected using a Westar Supernova kit (Cyanagen) with a Gel Imager System (Bio‐Rad)." (More...)

BMC Complement Med Ther    |    Jul 6, 2021    |    PubMed ID: 34229670    |    Read Article

Piperlongumine inhibits migration and proliferation of castration-resistant prostate cancer cells via triggering persistent DNA damage

Ding-Fang Zhang, Zhi-Chun Yang, ..., Xiao-Hui Zheng

"... (Santa), RPA (Santa), KU70 (Santa), XRCC4 (Santa) and β-actin (Proteintech) overnight at 4 °C.. Finally, the blots were detected with HRP-conjugated secondary antibodies and visualized using a Westar Supernova kit (Cyanagen).. Statistical analysis GraphPad Prism 5 was used for statistical analysis." (More...)

Cells    |    Jun 30, 2021    |    PubMed ID: 34209408    |    Read Article

Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells

Chinmaya Panda, Clara Voelz, ..., Alexander Slowik

"... Fusion Solo detection system (Peqlab, Darmstadt, Germany). lists the antibodies used for Western blot.. For chemiluminescence detection of bands, the Westar Supernova (Cyanagen, Bologna, Italy) substrate reagent was used.. Using ImageJ software (NIH, Bethesda, MD, USA), densitometric analysis of bands was performed and ..." (More...)

Pharmaceutics    |    Jun 23, 2021    |    PubMed ID: 34201872    |    Read Article

The Stimulating Effect of Rosmarinic Acid and Extracts from Rosemary and Lemon Balm on Collagen Type I Biosynthesis in Osteogenesis Imperfecta Type I Skin Fibroblasts

Joanna Sutkowska, Natalia Hupert, ..., Anna Galicka

"... incubated for 1 h at room temperature with slow mixing.. After washing (3 times for 10 min with TBS-T) membrane was developed using a Westar Supernova Chemiluminescent Substrate for Western Blotting (Cyanagen, Bologna, Italy) and documented with the use of BioSpectrum Imaging System gel documentation apparatus (UVP, Upland, CA, USA).. The intensity of the protein bands was measured by densitometry, ..." (More...)

Sci Rep    |    Jun 3, 2021    |    PubMed ID: 34083607    |    Read Article

Composition and function of the C1b/C1f region in the ciliary central apparatus

Ewa Joachimiak, Anna Osinka, ..., Dorota Wloga

"... (1:2000), rabbit monoclonal anti-V5 (1:1600), and mouse monoclonal anti-α-tubulin (12G10) (1:40,000) at 4 °C.. After washing (4 × 10 min in TBST) membranes were incubated with the appropriate HRP-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA, USA) for 1 h at RT, washed and proteins were detected using Westar Supernova kit (Cyanagen, Italy).. For analyses of the biotinylated ciliary protein, samples were run on the 10% SDS-PAGE ..." (More...)

Front Immunol    |    May 13, 2021    |    PubMed ID: 34054836    |    Read Article

Interferon Gamma Induces the Increase of Cell-Surface Markers (CD80/86, CD83 and MHC-II) in Splenocytes From Atlantic Salmon

Byron Morales-Lange, Felipe Ramírez-Cepeda, ..., Luis Mercado

"... Thermo) was used diluted 1:5,000 in 1% BSA for 60 min at room temperature.. Specific bands were visualized by chemiluminescence with the Westar Supernova kit (Cyanagen).. Immunofluorescence analyses were carried out using control and Ss rIFNγ-induced splenocytes after 15 days ..." (More...)

Medical Oncology    |    Apr 21, 2021    |    Read Article

H2O2 enhances the anticancer activity of TMPyP4 by ROS-mediated mitochondrial dysfunction and DNA damage

Jianqiang Chen, Xiangxiang Jin

"Abstract: Cancer is one of the diseases that threatens human health and is a leading cause of mortality worldwide. High levels of reactive oxygen species (ROS) have been observed in cancer tissues compared with normal tissues in vivo, and it is not yet known how this influences chemotherapeutic drug action" (More...)

Cells    |    Mar 1, 2021    |    Read Article

Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells

Abhirup Bagchi, Aneesha Nath, ..., Shaji R Velayudhan

"... Technologies, Danvers, MA, USA) and anti-rabbit IgG HRP (Invitrogen Corporation, Camarillo, CA, USA). ... The signal was detected using the Westar Supernova (Cyanagen, Bologna, Italy) and FluorChemE gel documentation system (Protein Simple, San Jose, CA, USA).. WESTAR SUPERNOVA , xls3 , xls3 , westar supernova , Westar Supernova ," (More...)

Biology of Reproduction    |    Feb 8, 2021    |    Read Article

Activation of bovine oocytes by protein synthesis inhibitors: new findings on the role of MPF/MAPKs†

Cecilia Valencia, Felipe Alonso Pérez

"Abstract: The present study evaluated the mechanism by which protein synthesis inhibitors activate bovine oocytes. The aim was to analyze the dynamics of MPF and MAPKs" (More...)

bioRxiv    |    Jan 27, 2021    |    Read Article

Application of the SMALP technology to the isolation of GPCRs from low-yielding cell lines

Tedesco Daniele, Maj Maciej, Zaffagnini Mirko

"PrePrint: The Westar Supernova substrate for enhanced chemiluminescence (ECL) detection (Cyanagen, Italy) was used to visualize the membranes; ECL was recorded using an Azure c400 western blot imaging system (Azure Biosystems, USA) with an exposure time of 1800 and 3600 s for membranes treated with anti-6×His and anti-FLAG M2 primary antibodies, respectively." (More...)

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	K-XLSE2-ELISTAR Supernova超级ECL发光液	操作手册
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