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Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
Nicholson, D.W. et al. (1995) Nature 376, 37-43.
Product Usage Information
Application Dilution Western Blotting 1:1000 Simple Western™ 1:50 - 1:250 Immunoprecipitation 1:50 Immunohistochemistry (Paraffin) 1:1000 - 1:4000 Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized) 1:800 - 1:3200 REACTIVITY H M R Mk SENSITIVITY Endogenous MW (kDa) 17, 19 Source/Isotype Rabbit IgG Application Key:
WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence
F-Flow Cytometry
Species Cross-Reactivity Key:
H-Human
M-Mouse
R-Rat
Mk-Monkey
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产品组分
内容
型号
规格 储存温度
Cleaved Caspase-3 (Asp175) (5A1E) 兔单克隆抗体
9664T 20ul -20°C Cleaved Caspase-3 (Asp175) (5A1E) 兔单克隆抗体
9664S 100ul -20°C Cleaved Caspase-3 (Asp175) (5A1E) 兔单克隆抗体
9664L 300ul -20°C 操作手册
1 1 常温室温
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9664-Cleaved Caspase-3 (Asp175) (5A1E) 兔单克隆抗体
操作手册 9664-Cleaved Caspase-3 (Asp175) (5A1E) 兔单克隆抗体相关单页
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Journal:
Neural Regen Res
Title:
Author:
Yumei Liao, et al.
Year:
2026
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
Journal:
Int J Mol Med
Title:
Author:
Chuanxi Lai, et al.
Year:
2026
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Mol Ther Nucleic Acids
Title:
Author:
Pinja Kettunen, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Apoptosis
Title:
Author:
Xiang-Yu Han, et al.
Year:
2025
Applications:
Unspecified
Reactivity:
Unspecified
Journal:
Mol Biol Cell
Title:
Author:
Janakiram R Vangala, et al.
Year:
2025
Applications:
Western Blotting (WB)
Reactivity:
Unspecified
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