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过氧化氢酶(CAT)检测试剂盒

过氧化氢酶(CAT)检测试剂盒,Catalase Assay Kit,用于测定血浆、血清、红细胞裂解物、组织匀浆和细胞裂解物中的过氧化氢酶(CAT)活性。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。


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过氧化氢酶(CAT)检测试剂盒



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CN707002

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  • 过氧化氢酶(EC 1.11.1.6;2H₂O₂氧化还原酶)是一种普遍存在的抗氧化酶,存在于大多数需氧细胞中。过氧化氢酶(CAT)参与过氧化氢(H₂O₂)的解毒过程,过氧化氢是一种活性氧(ROS),是正常有氧代谢和致病性活性氧产生的有毒产物。这种酶催化两分子过氧化氢转化为分子氧和两分子水(催化活性)。CAT还具有过氧化物酶活性,其中低分子量醇可作为电子供体。虽然脂肪醇是CAT的特异性底物,但其他具有过氧化物酶活性的酶并不利用这些底物。在人体中,过氧化氢酶的最高水平存在于肝脏、肾脏和红细胞中,据信它在过氧化氢的分解中占大部分。过氧化氢酶检测试剂盒利用CAT的过氧化物酶功能来测定酶活性。该方法基于酶与甲醇在最适浓度的H₂O₂存在下的反应。生成的甲醛用4-氨基-3-肼基-5-巯基-1,2,4-三唑(Purpald)作为显色剂通过分光光度法测定。1,2 Purpald与醛类特异性形成双环杂环,氧化后由无色变为紫色。1,2 该检测可用于测量血浆、血清、红细胞裂解物、组织匀浆和细胞裂解物中的CAT活性。

    过氧化氢酶(CAT)检测试剂盒具有如下优势和特性:

    • 测定血浆、血清、红细胞裂解液、组织匀浆和细胞裂解液中的过氧化氢酶活性
    • 对40个样本进行双份检测
    • 可检测低至2纳摩尔/分钟/毫升的过氧化氢酶活性
    • 基于平板的比色法测定(540纳米)
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    过氧化氢酶(CAT)检测试剂盒

    CN707002-01

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    -20°C

    过氧化氢酶(CAT)检测试剂盒

    CN707002-02

    480次

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    保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

     

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  • Product Description References

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    2. Wheeler, C.R., Salzman, J.A., Elsayed, N.M., et al. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Anal. Biochem. 184, 193-199 (1990).

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