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hMeDIP试剂盒【针对纯化好的DNA】
当胞嘧啶环的5-Carbon处的甲基组共价时, 产生5-甲基胞嘧啶,DNA就发生甲基化。这些甲基组投入DNA深沟中,并抑制转录。在人类DNA中,5-甲基胞嘧啶占DNA全基因组大约1.5%,主要在CpG处。0.3 至0.2 kb DNA段中CpG簇,称之为CpG岛,这些CpG岛典型性的出现在基因启动子区或者在其附近,在该处转录开始。在大量基因组DNA中,大部分CpG段都被重度甲基化。然而,在处于萌芽阶段的组织及正常体细胞的启动子中,CpG岛仍然保持未被甲基化,可以产生基因表达。当CpG岛在基因启动子区已经甲基化了,该基因的表达就被抑制。这种抑制起因于直接抑制特异转录因子结合,及间接充实甲基-CpG-结合蛋白及其相关抑制染色质重组装活动。除对基因转录的影响外,DNA甲基化还影响到染色质结构域的形成和基因组印记,该印记引用亲本基因的原特异表达式。 最近,一种新的修饰的核苷酸被命名为:5-hmC,且在老鼠的大脑和胚胎干细胞中已经发现。在哺乳动物中,它是由甲基化胞嘧啶氧化所产生的,这是一个通过T族酶和DNA甲基转移酶蛋白介导的一个反应 。下面是胞嘧啶的甲基化、羟甲基化形式:
产品组分
组分内容 |
型号:A-P-1038-24(24次) |
型号:A-P-1038-48(48次) | 型号:A-P-1038-96(96次) |
储存温度 |
WB(10X Wash Buffer) |
5 ml | 10 ml | 20 ml |
4°C
|
AB(Antibody Buffer) | 4 ml | 8 ml | 16 ml | 室温 |
HS(hMeDIP Solution) | 3 ml | 6 ml | 12 ml | 室温 |
DRB(DNA Release Buffer) | 7 ml | 14 ml | 28 ml | 室温 |
Non-Immune IgG(0.6mg/ml)* | 10 ul | 20 ul | 40 ul | 4°C |
5-hmC Antibody(0.6mg/ml)* | 25 ul | 50 ul | 100 ul | 4°C |
Control DNA(500ng/ml)* | 5 ul | 10 ul | 20 ul | -20°C |
Normal-Mouse IgG(1mg/ml)* | 10 ul | 20 ul | 40 ul | 4°C |
Proteinase K(10mg/ml)* | 28 ul | 56 ul | 112 ul | 4°C |
Control Primer-Forward(20uM)* | 5 ul | 10 ul | 20 ul | 4°C |
Control Primer-Reverse(20uM)* | 5 ul | 10 ul | 20 ul | 4°C |
8-Well Assay Strips(With Frame) | 3条 | 6条 | 6条 | 4°C |
Adhesive 8-Well Strips Film | 3片 | 6片 | 6片 | 室温 |
操作手册 |
1 |
1 |
室温 |
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保存建议 | 推荐蓝冰或冰袋运输。当您收到产品后,按照说明书建议保存各组分。 |
警告 | 本品仅供科研使用,请勿用于临床与诊断。 |
FAQ
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