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细胞黏附是一个复杂的过程，涉及迁移/侵袭、胚胎发生、伤口愈合和组织重塑。细胞黏附于细胞外基质，与细胞骨架成分形成复合物，这些复合物会影响细胞的运动性、分化、增殖和存活。
48孔ECM阵列细胞粘附比色检测试剂盒，细胞外基质阵列。将三种不同细胞系的血清饥饿细胞以100,000个细胞/孔的密度接种到包被有细胞外基质的48孔板中，孵育1小时。根据测定方案对贴壁细胞进行染色。BSA=牛血清白蛋白。VN=玻连蛋白。LN=层粘连蛋白。FN=纤连蛋白。Col=胶原蛋白。我们的48孔ECM阵列细胞粘附比色检测试剂盒提供了一种完全定量的方法来评估细胞粘附。48孔板的前五排每孔均预包被您选择的单一细胞外基质蛋白，最后一排作为阴性对照。只需遵循以下简单步骤即可：
将细胞接种到基质上。
贴壁细胞会附着，而非贴壁细胞会被冲洗掉。
使用标准酶标仪或荧光计对贴壁细胞进行定量。
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48孔ECM阵列细胞粘附比色检测试剂盒
CBA070-01 48次 -20°C 48孔ECM阵列细胞粘附比色检测试剂盒
CBA070-02 240次 -20°C 操作手册
1 1 常温
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保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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- Potter, J.A. et al. (2021). Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin α2β1 Binding in TGFβ1-Evoked Renal Tubular Epithelial Cells. Int J Mol Sci. 22(7):3644. doi: 10.3390/ijms22073644 (#CBA-070).
- Liu, S.Y. et al. (2021). Evaluation of Nanotargeted 111In-Cyclic RGDfK-Liposome in a Human Melanoma Xenotransplantation Model. Int J Mol Sci. 22(3):1099. doi: 10.3390/ijms22031099 (#CBA-070).
- Iosef, C. et al. (2020). Quantitative proteomics reveal lineage-specific protein profiles in iPSC-derived Marfan syndrome smooth muscle cells. Sci Rep. 10(1):20392. doi: 10.1038/s41598-020-77274-w (#CBA-070).
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- Celentano, A. et al. (2020). Kava constituents exert selective anticancer effects in oral squamous cell carcinoma cells in vitro. Sci Rep. 10(1):15904. doi: 10.1038/s41598-020-73058-4 (#CBA-070).
- Jha, S.K. et al. (2020). Enhanced antitumor efficacy of bile acid-lipid complex-anchored docetaxel nanoemulsion via oral metronomic scheduling. J Control Release. 328:368-394. doi: 10.1016/j.jconrel.2020.08.067 (#CBA-070).
- Kanda, M. et al. (2020). Therapeutic monoclonal antibody targeting of neuronal pentraxin receptor to control metastasis in gastric cancer. Mol Cancer. 19(1):131. doi: 10.1186/s12943-020-01251-0 (#CBA-071).
- Bagdonaite, I. et al. (2020). O-glycan initiation directs distinct biological pathways and controls epithelial differentiation. EMBO Rep. doi: 10.15252/embr.201948885 (#CBA-070).
- Okada, J. et al. (2020). Dapagliflozin Inhibits Cell Adhesion to Collagen I and IV and Increases Ectodomain Proteolytic Cleavage of DDR1 by Increasing ADAM10 Activity. Molecules. 25(3). pii: E495. doi: 10.3390/molecules25030495 (#CBA-070).
- Nakamura, S. et al. (2020). KCNJ15 Expression and Malignant Behavior of Esophageal Squamous Cell Carcinoma. Ann Surg Oncol. doi: 10.1245/s10434-019-08189-8 (#CBA-071).
- Liu, L.Q. et al. (2019). MiR-92a antagonized the facilitation effect of extracellular matrix protein 1 in GC metastasis through targeting its 3'UTR region. Food Chem Toxicol. 133:110779. doi: 10.1016/j.fct.2019.110779 (#CBA-070).
- Hou, S. et al. (2019). Integrin α5 promotes migration and cisplatin resistance in esophageal squamous cell carcinoma cells. Am J Cancer Res. 9(12):2774-2788 (#CBA-070).
- Uno, Y. et al. (2019). Expression, Function, and Prognostic Value of MAGE-D4 Protein in Esophageal Squamous Cell Carcinoma. Anticancer Res. 39(11):6015-6023. doi: 10.21873/anticanres.13807 (#CBA-070).
- Umeda, S. et al. (2019). Fraser extracellular matrix complex subunit 1 promotes liver metastasis of gastric cancer. Int J Cancer. doi: 10.1002/ijc.32705 (#CBA-070).
- Hirano, K. et al. (2019). LacdiNAcylation of N-glycans in MDA-MB-231 human breast cancer cells results in changes in morphological appearance and adhesive properties of the cells. Histochem Cell Biol. doi: 10.1007/s00418-019-01822-3 (#CBA-070).
- Ding, L. et al. (2019). Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. Nat Commun. 10(1):4182. doi: 10.1038/s41467-019-12125-5 (#CBA-070).
- Birtley, J.R. et al. (2019). Inactivating mutations and X-ray crystal structure of the tumor suppressor OPCML reveal cancer-associated functions. Nat Commun. 10(1):3134. doi: 10.1038/s41467-019-10966-8 (#CBA-070).
- Sawaki, K. et al. (2019). Level of Melanotransferrin in Tissue and Sera Serves as a Prognostic Marker of Gastric Cancer. Anticancer Res. 39(11):6125-6133. doi: 10.21873/anticanres.13820 (#CBA-071).
- Maxwell, J.T. et al. (2019). Electrical stimulation of pediatric cardiac-derived c-kit+ progenitor cells improves retention and cardiac function in right ventricular heart failure. Stem Cells. doi: 10.1002/stem.3088 (#CBA-071).
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- Zohra, F. T. et al. (2015). Functional behavior and gene expression of magnetic nanoparticle-loaded primary endothelial cells for targeting vascular stents. Nanomedicine (Lond). 10:1391-1406 (#CBA-071).
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