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For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C11H13N3O4
Molecular Weight: 251.24 g/mol
Exact Mass: 251.09 g/mol
CAS#: 69075-47-4
Purity: ≥ 99 % (HPLC)
Form: solid
Color: off-white
Solubility: DMSO
Spectroscopic Properties: λmax 291 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
DNA synthesis monitoring[1]Description:
Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2’-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine.
The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to
introduce a Biotin group (Azides of Biotin) for subsequent purification
tasks or a fluorescent group (Azides of fluorescent dyes) for subsequent
microscopic imaging [1].Presolski et al.[2] and Hong et al.[3] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
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产品组分
内容
型号
规格 储存温度
5-乙炔基-2'-脱氧胞苷(5-EdC)
CLKN003-01 10mg
-20 °C
5-乙炔基-2'-脱氧胞苷(5-EdC)
CLKN003-02 20mg
-20 °C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Guan et al. (2011) Intracellular detection of cytosine incorporation in genomic DNA by using 5-ethynyl-2'-deoxycytidine. ChemBioChem 2 (14):2184.
[2] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[3] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.MX2 forms nucleoporin-comprising cytoplasmic biomolecular condensates that lure viral capsids.
George D Moschonas, Louis Delhaye, ..., Xavier Saelens
Cell host & microbe | 2024 Oct 10 | 39389033
DNA Replication across α- l -(3′-2′)-Threofuranosyl Nucleotides Mediated by Human DNA Polymerase η
Rachana Tomar, Pratibha P. Ghodke, ..., Martin Egli
Biochemistry | 2024 Sep 11 | 39259676
Replication Bypass of the N -(2-Deoxy- d -erythro-pentofuranosyl)-urea DNA Lesion by Human DNA Polymerase η
Rachana Tomar, Songlin Li, ..., Michael P. Stone
Biochemistry | 2024 Feb 27 | 38413007
Genome-wide measurement of DNA replication fork directionality and quantification of DNA replication initiation and termination with Okazaki fragment sequencing.
Xia Wu, Yaqun Liu, ..., Nataliya Petryk
Nature protocols | 2023 Apr 13 | 36653528
Monitoring genome-wide replication fork directionality by Okazaki fragment sequencing in mammalian cells
Sarah Kit Leng Lui, Sarah Keegan, ..., Tony T. Huang
Nature Protocols | 2022 Jan 27 | 33442052
The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex.
Michael Bauer, Justin W Flatt, ..., Urs F Greber
Cell reports | 2020 Sep 24 | 31851912
Template-assisted synthesis of adenine-mutagenized cDNA by a retroelement protein complex
Sumit Handa, Yong Jiang, ..., Partho Ghosh
Nucleic Acids Research | 2018 Oct 12 | 30007279
Molecular Dissection of Chromatin Maturation Via Click Chemistry
Ozlem Yildirim, Robert E Kingston
Current protocols in molecular biology / e… | 2016 Apr 01 | 27038388
Induced Fit in the Selection of Correct versus Incorrect Nucleotides by DNA Polymerase β
Beth Moscato, Monalisa Swain, J. Patrick Loria
Biochemistry | 2015 Dec 30 | 26678253
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