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For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C10H13N5O5
Molecular Weight: 283.24 g/mol
Exact Mass: 283.09 g/mol
Purity: ≥ 90 % (HPLC)
Form: solid
Color: white to off-white
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 263 nm, ε 9.7 L mmol-1 cm-1
Applications:
DNA synthesis monitoring[1]Description:
5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.
5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine.
The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice to- introduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly)
- introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).
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产品组分
内容
型号
规格 储存温度
5-叠氮甲基-2'-脱氧尿苷(5-AmdU)
CLK064-01 10mg
-20 °C
5-叠氮甲基-2'-脱氧尿苷(5-AmdU)
CLK064-02 20mg
-20 °C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Neef et al. (2014) An azide-modified nucleoside for metabolic labeling of DNA. Chembiochem. 15 (6):789.
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