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For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C31H28N4O7 (free acid)
Molecular Weight: 568.58 g/mol (free acid)
Exact Mass: 568.20 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solid
Color: colorless to slightly yellow
Solubility: DMSO (up to 200 mM tested)
Spectroscopic Properties: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
DNA synthesis monitoring (potentially)Description:
5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells.
5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine.The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to
- introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
- introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes).
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产品组分
内容
型号
规格 储存温度
5-二苄基环辛炔-2'-脱氧尿苷(5-DBCO-dU)
CLK082-01 10mg
-20 °C
5-二苄基环辛炔-2'-脱氧尿苷(5-DBCO-dU)
CLK082-02 20mg
-20 °C 操作手册
1 1 常温
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CLK082-5-二苄基环辛炔-2'-脱氧尿苷(5-DBCO-dU)
中文手册 CLK082-5-二苄基环辛炔-2'-脱氧尿苷(5-DBCO-dU)相关单页
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Neef et al. (2014) An azide-modified nucleoside for metabolic labeling of DNA. Chembiochem. 15 (6):789.
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