5-乙烯基-2'-脱氧尿苷(5-VdU)
5-乙烯基-2'-脱氧尿苷(5-VdU)，5-Vinyl-2'-deoxyuridine (5-VdU)，5-Ethenyl-2'-deoxyuridine，5-乙烯基-2'-脱氧尿苷，2'-Deoxy-5-vinyluridine，2'-脱氧-5-乙烯基尿苷，2'-Deoxy-5-ethenyluridine，2'-脱氧-5-乙烯基尿苷，具有细胞渗透性，会取代其天然类似物胸苷并入正在复制的DNA中。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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5-乙烯基-2'-脱氧尿苷(5-VdU)
所属分类
目录号
CLK050
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For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C11H14N2O5
Molecular Weight: 254.24 g/mol
Exact Mass: 254.09 g/mol
CAS#: 55520-67-7
Purity: ≥ 98 % (HPLC)
Form: solid
Color: white to off-white
Solubility: DMSO, methanol
Spectroscopic Properties: λmax 292 nm, ε 9.2 L mmol-1 cm-1 (Methanol)
Applications:
DNA synthesis monitoring[1]Description:
5-VdU (5-Vinyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle.5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging [1].
[1] Salic et al. (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc.Natl. Acad. Sci. USA 105: 2415.
[2] Hamelik et al. (2009) Click-iTTM Assay with Improved DNA Distribution Histograms. Cytometry A 75A:862.
[3] Diermeier-Daucher et al. (2009) Cell Type Specific Applicability of 5-Ethynyl-2’-deoxyuridine (EdU) for Dynamic Proliferation Assessment in Flow Cytometry. Cytometry A 75A:535
[4] Buck et al. (2008) Detection of S-Phase cell cycle progression using 5’-ethynyl-2’-deoxyuridine incorporation with click chemistry, an alternative to using 5’-bromo-2’-deoxyuridine. Biotechniques 44(7):927.
[5] Limsirichaikul et al. (2009) A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU). Nucleic Acids Res. 37(4):e31.
[6] Zeng et al. (2010) Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Research. 1319:21.
[7] Warren et al. (2009) Chick embryo proliferation studies using EdU labeling. Dev Dyn. 238(4):944.[8] Monzack et al. (2012) A time course investigation of the statin paradox among valvular interstitial cell phenotypes. American Journal of Physiology. 303(7):H903.
[9] Gouge et al. (2010) Detection of S Phase in multiple Drosophila tissues utilizing the EdU labeling technique. Dros. Inf. Serv. 93: 213.
[10] Kotogány et al. (2010) A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Plant Methods 6: 5.
[11] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53: 9168. -
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规格 储存温度
5-乙烯基-2'-脱氧尿苷(5-VdU)
CLK050-01 10mg
-20 °C
5-乙烯基-2'-脱氧尿苷(5-VdU)
CLK050-02 50mg
-20 °C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Rieder et al. (2014) Alkene-Tetrazine Ligation for Imaging Cellular DNA. Angew. Chem. Int. Ed. 53:9168.Supplementary The trans cell cycle effects of PARP inhibitors underlie their selectivity toward BRCA1/2-deficient cells
Antoine Simoneau, Rosalinda Xiong, Lee Zou
Genes & Development | 2021 Sep 01 | 34385259
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