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For general laboratory use. Not intended for human or animal diagnostic or therapeutic uses.
Shipping: Shipped at ambient temperature/on gel packs
Storage Conditions: store components as indicated on individual labels
Shelf Life: 12 months
Description:
All-inclusive kit for preparation of solidified medium for clonal selection of Leishmania tarentolae.Content:
- 2 x 250 ml of Agar base
- 400 ml of buffered liquid medium:
This component is provided in excess to allow for individual batches. - 2 x 50 ml heat-inactivated fetal calf serum (iFCS)
- 2 ml of Hemin stock solution, 500x (Cat.-No. ML-108S):
0,25% solution of Hemin in 30% Triethanolamine, sterile - 5 ml of Pen-Strep stock solution, 200x (Cat.-No. ML-105S):
10.000 units of penicillin (base) and 10.000 μg of streptomycin (base)/ml as penicillin G sodium salt and streptomycin sulfate in 0.85% saline, sterile - 40 pcs. Nitrocellulose membranes diameter 82 mm:
for easy plating, fluorescence monitoring and transfer of colonies to alternative conditions (e.g. induction of the LEXSY T7 polymerase-based gene expression) - 40 pcs. Petri dishes diameter 90 mm
- 40 pcs. Plating spatula in single bags
- Serological pipettes: 4 x 25 ml, 2 x 10 ml, 2 x 2 ml
All components are provided sterile.
Storage conditions:
- Agar base:
Store at room temperature
Stable for 12 months - Buffered liquid medium:
Store at 4 °C
Stable for 12 month - iFCS:
Store < -20 °C
Stable for 12 month - Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month - Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month
Preparation of 500 ml LEXSY plating medium (20 plates, each 25 ml) :
- Microwave-melt 250 ml Agar base in provided glass flask and keep at 55 °C.
- Add pre-warmed (37 °C) buffered liquid medium to ca. 400 ml.
- Add 50 ml iFCS, 1 ml Hemin stock and 2.5 ml Pen-Strep.
- Fill with buffered liquid medium to 500 ml.
- Add appropriate antibiotics (not provided).
- Pour plates with 25 ml pre-warmed (55 °C) serological pipette, avoid air bubbles.
- After solidification, dry the plates open under the laminar flow box for exactly 10 min, than store them closed bottom-up.
- After plating, seal plates with parafilm and incubate bottom- up for 5 - 9 days at 26 °C until colonies appear.
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产品组分
组分内容
型号 规格 储存温度
利什曼原虫舒适型电镀试剂盒
ML451-01 40块 -20°C 利什曼原虫舒适型电镀试剂盒
ML451-02 80块 -20°C 操作手册
1 1 室温
*注意：在使用之前，应将溶液离心至管底。
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营销中心
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注意事项
保存建议 推荐蓝冰或冰袋运输。当您收到产品后，按照说明书建议保存各组分。 警告 本品仅供科研使用，请勿用于临床与诊断。 -
FAQ
1. RT-PCR反应后，无PCR产物或出现Smear，怎么办？
此时请从提取的RNA样品的纯度和添加量、基因的表达丰度、基因的长度和GC含量、引物的设计情况、参考文献的可信度以及RT-PCR条件的设定等方面加以考虑。
2. PCR反应时，反转录反应液的使用量使用多少较为合适？
反转录反应液的使用量可控制在1 μl～10 μl的范围。
3. 3′RACE PCR扩增产物经电泳分析后，有时为什么出现多条带现象？
可能是因为未知序列信息不清楚导致了非特异性扩增； 扩增的基因为多基因家族的成员；mRNA不同的拼接方式造成的。
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