PEP2-8

PEP2-8，是 PCSK9 抑制剂，其 KD 值为 0.7 μM，IC50 值为1.4 μM。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

关键词

DOPE

所属分类

目录号

HY-P2276

Pep2-8 是 PCSK9 抑制剂，其 K_D 值为 0.7 μM，IC₅₀ 值为1.4 μM。

生物活性

Pep2-8 is a PCSK9 inhibitor with a binding K_D of 0.7 μM and an IC₅₀ of 1.4 μM^[1].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HepG2	EC₅₀	6 μM	Reversal of LDL uptake in human HepG2 cells for 2 hrs preincubation with PCSK9 followed by LDL addition and measured after 4 hrs by fluorimetry method relative to control	[PMID: 34266235]
LAD2	EC₅₀	> 5 μM	Effect on mast cell degranulation in human LAD2 cells assessed as CCL3 release measured after 2 hrs by Luminex bead based assay	[PMID: 34266235]

体外研究
(In Vitro)

Pep2-8 与 C 末端截短的 PCSK9^[1]结合。
在 50 μM 的浓度下，Pep2-8 将 PCSK9 处理的 HepG2 细胞的 LDL 摄取恢复到对照活性的约 90%^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

体内研究
(In Vivo)

Pep2-8 与 C 末端截短的 PCSK9^[1]结合。
在 50 μM 的浓度下，Pep2-8 将 PCSK9 处理的 HepG2 细胞的 LDL 摄取恢复到对照活性的约 90%^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1715.85

Formula

C₈₃H₁₁₀N₁₆O₂₄

CAS 号

性状

固体

颜色

White to off-white

Sequence

Ac-Thr-Val-Phe-Thr-Ser-Trp-Glu-Glu-Tyr-Leu-Asp-Trp-Val-NH2

Sequence Shortening

Ac-TVFTSWEEYLDWV-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

产品组分

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注意事项

保存建议厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。
FAQ
[1]. Yingnan Zhang, et al. Identification of a small peptide that inhibits PCSK9 protein binding to the low density lipoprotein receptor. J Biol Chem. 2014 Jan 10;289(2):942-55.