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单链选择性单功能尿嘧啶-DNA 糖基化酶，亦称 SMUG1，是一种负责识别基因组中碱基损伤并启动碱基切除DNA修复的酶。该蛋白作为单功能DNA糖基化酶，对DNA中的尿嘧啶（U）残基具有特异性，且优先结合单链DNA底物。
Source
Recombinant protein corresponding to aa1-270 from human SMUG1 at N-terminal, fused to His-tag expressed in E.coli.
Molecular Weight
~32.3kD (293aa)
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
≥95% (SDS-PAGE)
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 1mM DTT, 30% glycerol.
Grade
Highly Purified
Molecular Weight
32.3
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C
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产品组分
内容
型号
规格 储存温度
带His标签重组人SMUG1氨基酸1-270 138744-01 100ug -20°C 带His标签重组人SMUG1氨基酸1-270 138744-02 500ug -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
1. Masaoka A., et al. (2003) Biochemistry. 42:5003-5012 2. Boorstein R.J., et al. (2001) J. Biol. Chem. 276:41991-41997.
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