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染料修饰的脱氧尿苷三磷酸（如氨基烯丙基-dUTP）可通过常规酶促掺入方法（如逆转录、缺口平移、随机引物标记或PCR）用于生产含染料的DNA。这种酶促荧光标记技术广泛应用于FISH探针和微阵列实验。
iFluor®555-PEG12-dUTP偶联物与ChromaTide™Alexa Fluor 555-dUTP具有完全相同的光谱特性，其PEG12间隔基能显著提高聚合酶对底物的掺入效率。作为红色荧光标记物，该染料适配SpectrumOrange™滤光片系统，可提供亮度高、光稳定性强且不受pH值影响的荧光信号。ChromaTide™与Alexa Fluor™是ThermoFisher公司的注册商标，SpectrumOrange™是Vysis公司的注册商标。
产品优势
1.标记兼容性：支持逆转录、PCR及缺口平移等多种技术
2.明亮红色信号：与SpectrumOrange™滤光片系统兼容，光稳定性强，且不受pH值影响
适用范围
应用于FISH探针和微阵列实验。
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产品组分
内容
型号
规格 储存温度
iFluor 555-PEG12-dUTP 1 mM TE缓冲液(pH 7.5) 17042-01 25 nmoles -20°C iFluor 555-PEG12-dUTP 1 mM TE缓冲液(pH 7.5) 17042-02 100 nmoles -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors:
Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal:
Journal of agricultural and food chemistry (2020): 369-375
Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors:
Gutjahr, Alice and Xu, Shuang-yong
Journal:
Nucleic acids research (2014): e77
Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors:
Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal:
Analytical chemistry (2012): 5645-52
Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors:
Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal:
The Analyst (2011): 2252-9
Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors:
Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal:
Animal reproduction science (2011): 32-6
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