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人源MEKK2（亦称为MAP3K2和MEKK2B，GenBank登录号：NM_006609），全长序列，N端带有GST标签，分子量为96.5千道尔顿，通过杆状病毒表达系统在草地贪夜蛾9（Sf9）昆虫细胞中表达。
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity
≥87%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl , 20% glycerol and 3 mM DTT.
MW
96.5 kDa
Amino Acids
full length
Specific Activity
18 pmol/min/µg
Genbank #
NM_006609
UniProt #
Q9Y2U5
Tag(s)
N-terminal GST-tag
Stability
>6 months at -80°C.
Assay Conditions
Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo Plus reagents (Promega Corporation, Madison, WI).
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles
Scientific Category
Kinase/Serine-Threonine
Regulatory Status
Research Use Only
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产品组分
内容
型号
规格 储存温度
GST标签重组人MEKK2蛋白 40122-01 10ug -20°C GST标签重组人MEKK2蛋白 40122-02 20ug -20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
1. Blank, J. L. et al. J. Biol. Chem. 271:5361-5368, 1996.
2. Hammaker, D. R. et al. J. Immun. 172:1612-1618, 2004.
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