重组人源转铁蛋白

重组人源转铁蛋白，Transferrin (TRF) Recombinant, Human CAS: 11096-37-0，来自人 TRF（P02787）、N 端带 His 标签的 Val361-Lys683 重组蛋白，在大肠杆菌中表达。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

关键词

重组人源转铁蛋白

所属分类

酶

目录号

157102

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转铁蛋白、TF、运铁蛋白、β-1 金属结合球蛋白、DKFZp781D0156、PRO1400、PRO1557、PRO2086、血清运铁蛋白前体、铁传递蛋白。

转铁蛋白是一种单链多肽糖蛋白，属于铁结合蛋白家族。其分子量为79.5千道尔顿，血清浓度范围为1800-2700毫克/升。该蛋白由肝脏合成，包含两个结构域，每个结构域均具有一个对三价铁离子具有高亲和力的可逆结合位点。铁通过血液和组织间液被转运至利用和代谢部位。铁-转铁蛋白复合物对血红蛋白的合成以及某些类型的细胞分裂至关重要。当机体出现铁缺乏和妊娠情况时，血清中转铁蛋白浓度会升高；而铁负荷过多、感染及炎症性疾病时，其浓度则会下降。

Amino Acid Sequence

VKWCALSHHE RLKCDEWSVN SVGKIECVSA ETTEDCIAKI MNGEADAMSL DGGFVYIAGK CGLVPVLAEN YNKSDNCEDT PEAGYFAIAV VKKSASDLTW DNLKGKKSCH TAVGRTAGWN IPMGLLYNKI NHCRFDEFFS EGCAPGSKKD SSLCKLCMGS GLNLCEPNNK EGYYGYTGAF RCLVEKGDVA FVKHQTVPQN TGGKNPDPWA KNLNEKDYEL LCLDGTRKPV EEYANCHLAR APNHAVVTRK DKEACVHKIL RQQQHLFGSN VTDCSGNFCL FRSETKDLLF RDDTVCLAKL HDRNTYEKYL GEEYVKAVGN LRK

Predicted Isoelectric Point

5.1

Predicted Molecular Weight

42.6kD

Accurate Molecular Weight

41kD as determined by SDS-PAGE reducing conditions

Applications

Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions

Optimal dilutions to be determined by the researcher.

Included Protein Molecular Weight Marker

168631: Unstained Protein Molecular Weight Marker, 10-70kD Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.

Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.

Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel

Mini gels: 3-5ul/well Large gel: 7ul/well The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels. Store at -20°C Stable for 6 months after receipt.

Storage and Stability

Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note

Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

Source

Recombinant, E. coli

Purity

≥95%

Form

Supplied as lyophilized powder from 10mM PBS, pH 7.4, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin 300. Reconstitute with sterile PBS, to a concentration of 0.1-1mg/ml. Do not vortex.

CAS Number

11096-37-0

Grade

Highly Purified

Swiss Prot

P02787

Molecular Weight

42.6

EU Commodity Code

30021019

Shipping Temp

Blue Ice

Storage Temp

-70ºC/-20°C
关键词:
- 重组人源转铁蛋白

产品组分

内容	型号	规格	储存温度
重组人源转铁蛋白)	157102-01	10ug	-20°C
重组人源转铁蛋白	157102-02	50ug	-20°C
重组人源转铁蛋白	157102-03	200ug	-20°C
操作手册	1	1	常温