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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C12H20N5O15P3 (free acid)
Molecular Weight: 567.23 g/mol (free acid)
Exact Mass: 567.02 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λ 260 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription (up to 1400 bp tested). For a comprehensive protocol please refer to PP-501 or PP-501-Cy3/Cy5.
3'-Azide-Labeling of T7 promotor-containing oligonucleotides[1,2,3]
The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively)
- to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively)
- to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins -
产品组分
内容 型号
规格 储存温度
5-叠氮-C3-UTP NU157-01 20ul(10mM) -20°C 5-叠氮-C3-UTP NU157-02 100ul(10mM) -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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