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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C11H15N2O15P3 (free acid)
Molecular Weight: 508.16 g/mol (free acid)
Exact Mass: 507.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription.
The resulting alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice- to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin)
- to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes)
- to crosslink the RNA to azide-functionalized biomolecules e.g.proteins
Presolski et al.[1] and Hong et al.[2] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
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产品组分
内容 型号
规格 储存温度
5-乙炔基-UTP(5-EUTP) CLKT08-01 5ul(100mM) -20°C 5-乙炔基-UTP(5-EUTP) CLKT08-02 25ul(100mM) -20°C 5-乙炔基-UTP(5-EUTP) CLKT08-03 50ul(100mM) -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[2] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.A distal CTCF-binding site drives MYC expression plasticity in a negative feed-forward loop
Gao Chenying, Martino Mirco, ..., Göndör Anita
bioRxiv | 2026 Jan 31
The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in some animals
Malin A. Kjosavik, Katherine L. P. Downham, ..., Kathrin Garschall
BMC Molecular and Cell Biology | 2025 Dec 26 | 41454242
mRNA poly(A)-tail length is a battleground for coronavirus–host competition
Latifkar Arash, Levdansky Yevgen, ..., Bartel David
bioRxiv | 2025 Oct 10
High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler.
Taras Sych, Jan Schlegel, ..., Erdinc Sezgin
Nature biotechnology | 2023 Jun 12 | 37308687
Patent DNA polymerase theta mutants, methods of producing these mutants, and their uses
Marc Delarue, Volahasina (Irina) Randrianjatovo Gbalou
us11384345 | 2022 Jul 12
Native molecule sequencing by nano-ID reveals synthesis and stability of RNA isoforms
Kerstin C. Maier, Saskia Gressel, ..., Björn Schwalb
Genome Research | 2020 Sep 01 | 32887688
Metabolic Labeling of RNAs Uncovers Hidden Features and Dynamics of the Arabidopsis Transcriptome
Emese Xochitl Szabo, Philipp Reichert, ..., Sascha Laubinger
The Plant Cell | 2020 Apr 01 | 32060173
Patent High temperature selection of nucleotide-supported carbohydrate vaccines and resulting glycosylated oligonucleotides
Isaac J. Krauss
us10378017 | 2019 Aug 13
Metabolic labeling of RNAs uncovers hidden features and dynamics of the Arabidopsis thaliana transcriptome
Szabo Emese Xochitl, Reichert Philipp, ..., Laubinger Sascha
bioRxiv | 2019 Mar 26
Enzymatic synthesis of random sequences of RNA and RNA analogues by DNA polymerase theta mutants for the generation of aptamer libraries
Irina Randrianjatovo-Gbalou, Sandrine Rosario, ..., Marc Delarue
Nucleic Acids Research | 2018 Jul 06 | 29788485
Characterization of SID-1-dependent and independent intergenerational RNA transport pathways in Caenorhabditis elegans
Wang Eddie, Hunter Craig P.
bioRxiv | 2017 Jun 26
Supplementary Nucleotidyl transferase assisted DNA labeling with different click chemistries
Marie-Luise Winz, Eva Christina Linder, ..., Andres Jäschke
Nucleic Acids Research | 2015 Sep 30 | 26013812
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