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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C10H15N8O13P3 (free acid)
Molecular Weight: 548.19 g/mol (free acid)
Exact Mass: 548.00 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 281 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into RNA by 3'-End Labeling with yeast Poly(A) Polymerase(yPAP)[1]
Identification of protein ATP-binding sites[2]
The resulting azide-functionalized RNA can subsequently be processed via Cu(I)-free (azide-DBCO) or Cu(I)-catalyzed (azide-alkyne) click chemistry that offers the choice
- to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively)
- to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively)
- to crosslink the RNA to azide- or alkyne functionalized biomolecules e.g.proteins -
产品组分
内容 型号
规格 储存温度
8-叠氮基-ATP NU155-01 20ul(10mM) -20°C 8-叠氮基-ATP NU155-02 100ul(10mM) -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Winz et al. (2012) Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry. Nucleic Acids Res 40(10):e78.
[2] Olcott et al. (1998) Localization and Characterization of Two Nucleotide-binding Sites on the Anaerobic Ribonucleotide Reductase from Bacteriophage T4. J. Biol. Chem. 273 (38):24853.
[3] Zayas et al. (2015) Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging. Bioconjug Chem. 26 (8):1519-32.The pseudokinase domains of guanylyl cyclase-A and -B allosterically increase the affinity of their catalytic domains for substrate.
Aaron B Edmund, Timothy F Walseth, ..., Lincoln R Potter
Science signaling | 2020 Mar 16 | 30696704
Characterization of P2X4 receptor agonists and antagonists by calcium influx and radioligand binding studies.
Aliaa Abdelrahman, Vigneshwaran Namasivayam, ..., Christa E Müller
Biochemical pharmacology | 2017 May 09 | 27867013
PhD Thesis Modes of Nutrient Transport Across the Chlamydia trachomatis Inclusion
Ruiz, Sara Irene
ruiz sara irene 2012 modes of nutrient tra... | 2012 Jan 01
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