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For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H16N2O
Molecular Weight: 276.33 g/mol
Exact Mass: 276.13 g/mol
CAS#: 1255942-06-3
Purity: ≥ 90 % (HPLC)
Form: solid
Color: slightly yellow to slightly orange
Solubility: DCM, DMF, DMSO, THF
Applications:
Protein-peptide conjugates
Peptide-small molecule conjugates
18F radiolabelling
Protein-oligonucleotide conjugates
Surface modificationDescription:
Simple carbonyl/carboxyl reactive dibenzylcyclooctyne. Applicable for the modification of surfaces and particles with terminal acids, active esters and aldehydes and for the introduction of dibenzylcyclooctyne into organic/inorganic compounds.Important Product Information
Do not use DTT, TCEP or β-mercaptoethanol, because they will reduce the azide.
Copper-free Click Reaction
- Prepare the azide-containing sample in reaction buffer.
- Add DBCO-protein conjugate to azide-containing sample.
- Recommendation: Add 1 mol equivalent of limiting protein to 1.5 - 3.0 mol equivalents of highest abundance protein.
- Incubate the reaction at room temperature for 2 - 4 hours or at 4 °C for 2 - 12 hours.
- The reaction is now ready for purification.
Troubleshooting
Problem: Low conjugation of DBCO and azide
- Possible reason: Suboptimal reaction conditions
- Increase incubation time
- Optimize conjugation conditions by altering molar excess
- Perform conjugation reactions at 37 °C
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产品组分
内容 型号
规格 储存温度
DBCO-胺 CLKA103-01 25mg -20°C DBCO-胺 CLKA103-02 100mg -20°C DBCO-胺 CLKA103-03 1g -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
Kuppevelt et al. (2017) Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions. Scientific Reports 7:14785.
Simon et al. (2012) Facile Double-Functionalization of Designed Ankyrin Repeat Proteins using Click and Thiol Chemistries. Bioconjugate Chem. 23 (2):279.
Zeng et al. (2012). 64Cu Core-Labeled Nanoparticles with High Specific Activity via Metal-Free Click Chemistry. ACS Nano. 6 (6):5209.
Arumugam et al. (2011). [18F]Azadibenzocyclooctyne ([18F]ADIBO): A biocompatible radioactive labeling synthon for peptides using catalyst free [3+2] cycloaddition. Bioorg. Med. Chem. Lett. 21:6987.
Campbell-Verduyn et al. (2011). Strain-Promoted Copper-Free Click Chemistry for 18F Radiolabeling of Bombesin. Angew. Chem. Int. Ed. 50:11117.
Debets et al. (2010) Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition. Chem. Commun. 46:97.18 F-labeling and initial in vivo evaluation of a Hitomi peptide for imaging tissue transglutaminase 2.
Uwe Ackermann, Luise Jäger, ..., Andrew M Scott
Nuclear medicine and biology | 2023 Mar 03 | 36502585
Fluorescent Polymer-AS1411-Aptamer Probe for dSTORM Super-Resolution Imaging of Endogenous Nucleolin.
Laura Fabre, Corentin Rousset, ..., Arnaud Favier
Biomacromolecules | 2022 Jun 14 | 35549176
Supplementary Versatile Route toward Hydrophobically Polymer-Grafted Gold Nanoparticles from Aqueous Dispersions.
Julian Sindram, Marcel Krüsmann, ..., Matthias Karg
The Journal of Physical Chemistry B | 2021 Aug 04 | 34260239
Polymer ligand binding to surface-immobilized gold nanoparticles: a fluorescence-based study on the adsorption kinetics
Julian Sindram, Matthias Karg
Soft Matter | 2021 Jan 01
Diversification of polyphosphate end-labeling via bridging molecules
Catherine J. Baker, Stephanie A. Smith, ..., Matthew Bogyo
PLoS ONE | 2020 Aug 21 | 32822431
Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions
Toin H. van Kuppevelt, Arie Oosterhof, ..., Willeke F. Daamen
Scientific Reports | 2017 Nov 01 | 29093576
Patent Bioconjugates of cyanine dyes
Xinshe Xu, Daniel R. Draney, Lael Cheung
us09408924 | 2016 Aug 09
Copper-free click chemistry for attachment of biomolecules in magnetic tweezers
Jorine M. Eeftens, Jaco van der Torre, ..., Cees Dekker
BMC Biophysics | 2015 Sep 25 | 26413268
Patent Fluorescent imaging with substituted cyanine dyes
Xinshe Xu, Daniel R. Draney, ..., William M. Volcheck
us09089603 | 2015 Jul 28
Construction of a peptide microarray for auto-anti- body detection.
Vincent Cosandey, Fabien Debrot, ..., Marc E Pfeifer
Chimia | 2013 Jan 08 | 23146271
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