细胞检测试剂盒

细胞检测试剂盒，LIVE-Step™ Cell Assay System，专为代谢活性、存活哺乳动物细胞的高通量、均一化、高灵敏度发光定量检测而设计。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。

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细胞检测试剂盒

所属分类

细胞生物学

目录号

82648

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在细胞培养中测定活细胞数量是多个生物领域的一项关键检测指标。传统上，台盼蓝手动计数是标准方法。尽管自动细胞计数器带来了便利，但它们并非适用于高通量应用的理想选择。基于酶的检测方法（如脱氢酶测定法）可提供更具可扩展性的解决方案。研究人员通过检测酶活性，能够间接评估细胞活力。基于ATP的发光检测法则是一种直接且灵敏的方法。采用热稳定萤火虫荧光素酶的该方法，可在较长时间内提供稳定的检测结果。与其他技术相比，基于ATP的发光检测通常具有更高的灵敏度、可靠性和更易操作性，非常适合高通量筛选。

细胞检测试剂盒专为代谢活性、存活哺乳动物细胞的高通量、均一化、高灵敏度发光定量检测而设计。它包含一种即用型单一溶液，可一步完成细胞裂解并培养物中三磷酸腺苷（ATP）含量的测定。该检测的线性范围可达超过100,000个悬浮细胞和50,000个贴壁细胞，决定系数（R2）大于0.99。信号稳定性超过5小时（半衰期T1/2 >300分钟），为实验提供灵活度。本产品兼容多种培养基配方及酚红存在的情况，且经多次冻融循环后仍保持稳定。

在裂解的代谢活性细胞释放的镁离子、氧气和三磷酸腺苷的存在下，荧光素会产生发光信号。所产生的信号与裂解前培养物中存在的代谢活性活细胞数量成正比。

向含有哺乳动物细胞的孔中加入细胞裂解液会导致细胞裂解，并产生图1所示的荧光素酶介导的反应。随后在发光检测仪中检测所产生的信号。该信号与裂解前培养物中代谢活性的活细胞比例成正比。

Materials Required But Not Supplied
- Clear-bottom, white multi-well tissue culture plates compatible with the instrument being used
- Mammalian cell culture of interest
- Cell culture medium
- Orbital Shaker
- Luminometer
- ATP Solution (Optional)
Stability

Reagents will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible.

Applications
- Determine metabolically active, viable cells values in mammalian culture by using a standard curve.
Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit for cell culture instructions. For all further questions, please contact us.

Contraindications
- The presence of organic solvents or other compounds in the culture can result in luciferase inhibition and impact the readings.
- If organic solvents or other compounds are used, it is recommended that an initial test is performed to determine the impact of the compound alone. For example, prepare a reaction containing LIVE-Step™ Cell Assay Solution, 1 µM ATP and the Diluent Reagent used to prepare the compound, and a reaction containing LIVE-Step™ Cell Assay Solution, 1 µM ATP and the compound of interest in Diluent Reagent at the maximum concentration to be tested. If a decrease in signal is observed, it may be necessary to perform a titration of the compound to assess the value at which no impact is seen, or to use a control for each concentration being tested.
- It is recommended that the edge wells of the plate are not used, to avoid temperature gradients that can affect the signal.
- ATP contamination should be avoided, by using aseptic technique while performing the assay and avoiding addition of extracellular ATP in the culture media.
- Avoid exposure of the reagent to light and excessive heat during storage.
Warnings

Protect from light. Avoid freeze/thaw cycles.
关键词:
- 细胞检测试剂盒