生物素-16-dCTP
生物素-16-dCTP，Biotin-16-dCTP，Biotin-16-Propargylamino-dCTP，生物素-16-炔丙基氨基-dCTP，γ-[N-(Biotin-6-amino-hexanoyl-6-aminobutanoyl)]-5-(3-propargylamino)-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt，γ-[N-(生物素-6-氨基己酰基-6-氨基丁酰基)]-5-(3-炔丙基氨基)-2'-脱氧胞苷-5'-三磷酸，三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。
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生物素-16-dCTP
所属分类
目录号
NU809BIO16
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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C32H51N8O17P3S (free acid)
Molecular Weight: 944.78 g/mol (free acid)
Exact Mass: 944.23 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in 10 mM Tris-HCl
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [1,2]
- Primer Extension with Klenow exo- [3]
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [4]
- Reverse Transcription with MMLV Reverse Transcriptase [5]Description:
Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine.Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/ 50% dCTP
Please note: The optimal final concentration of Biotin-16-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-16-dCTP/dTTP ratio is recommended.
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储存温度
生物素-16-dCTP
NU809BIO16-01 200ul（1mM） 2-8°C 生物素-16-dCTP
NU809BIO16-02 1ml（1mM） 2-8°C 操作手册 1 1 常温 -
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NU809BIO16-生物素-16-dCTP
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FAQ
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Selected References:
[1] Sugimoto et al. (1999) Isolation and mapping of a putative b subunit of human ATP synthase (ATP-BL) from human leukocytes. DNA Res. 6 (1):29.
[2] Sreedharan et al. (1995) Structure, expression, and chromosomal localization of the type I human vasoactive intestinal peptide receptor gene. Proc. Natl. Acad. Sci. U S A. 92 (7):2939.
[3] Yue et al. (1997) Apoptosis and necrosis in the newborn piglet brain following transient cerebral hypoxia-ischaemia. Neuropathol. Appl. Neurobiol. 23 (1):16.
[4] Zammetto et al. (2005) Unambiguous identification of expressed MAGE-A genes on a DNA microarry. Clin Chem 51:2420.
[5] Grimmond et al. (2001) Expression Profiling with DNA Microarray's: A User's Perspective and Guide. In: DNA Microarrays: Gene Expression Applications (Jordan). Springer Verlag Berlin Heidelberg.New Spatially resolved m 6 A profiling using m 6 A-ARTR-DBiT.
Yu Xiao, Zhiliang Bai, ..., Chuan He
Nature Methods | 2026 Jun 09 | 42265211
Super-resolved spatial organization of the nucleolar transcriptome
Fan Xinqi, Tan Chien-Chien, ..., Fei Jingyi
bioRxiv | 2026 May 21
Nuclear Speckles Regulate Splicing During Muscle Stem Cell Activation and Aging
Guzman Steve D., Duran Pamela, ..., Aguilar Carlos A.
bioRxiv | 2026 May 07
Multiscale Physical Effects of CpG Methylation on DNA Mechanics, Nucleosome Wrapping, and Chromatin Condensates
Daris LJC, Rizvi A, ..., Sanulli S
bioRxiv | 2026 Apr 14
Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments
Pavlina Gregorova, Minna-Maria K. Heinonen, ..., L. Peter Sarin
STAR Protocols | 2026 Mar 20 | 41653438
Quantifying the effect of magnesium ions on nucleosome stability in chromatin fibers.
Luuk J C Daris, Lieke A Lamers, ..., Jorine M Eeftens
Biophysical Journal | 2026 Mar 05 | 41793002
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