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人乙型肝炎病毒表面抗体(HBsAb)酶联免疫吸附测定试剂盒是一种高精度、高可靠性的检测工具，可用于检测人血清、血浆和细胞培养上清液中的乙肝表面抗体水平。该试剂盒具有高灵敏度和高特异性，能为研究人员的各类研究应用提供可靠且可重复的结果。乙肝表面抗体是一种关键抗体，在人体对乙肝病毒感染的免疫应答中发挥着核心作用。监测乙肝表面抗体水平对于评估人体对乙肝的免疫力以及评价疫苗效力至关重要。
该ELISA试剂盒助力研究人员研究乙型肝炎病毒的免疫应答，并制定乙型肝炎感染的预防与治疗策略。总体而言，人乙肝表面抗体（HBsAb）ELISA试剂盒是研究乙型肝炎病毒免疫学、疫苗开发及感染控制的不可或缺工具。其准确性与可靠性，为致力于进一步认识并对抗乙型肝炎感染的研究人员提供了宝贵助力。
Product Name: Human HBsAb (hepatitis B virus surface antibody) ELISA Kit Product Code: AEFI00444 Size: 96T Alias: HBsAb, hepatitis B virus surface antibody Detection method: Sandwich ELISA, Double Antigen Application: HBsAb ELISA Kit allows for the in vitro quantitative determination of HBsAb concentrations in serum, plasma, tissue homogenates and other biological fluids. Sensitivity: < 0.469ng/ml Range: 0.781-50ng/ml Storage: 2-8°C for 6 months Note: For Research Use Only Sample Type Protocol Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. Urine & Cerebrospinal Fluid Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. Cell culture supernatant Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. Cell lysates Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. Tissue homogenates The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. Tissue lysates Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. Breast Milk Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. -
产品组分
组分内容
型号 规格 储存温度
人乙型肝炎病毒表面抗体(HBsAb)酶联免疫吸附测定试剂盒
AEFI00444-01 96次 2-8°C 人乙型肝炎病毒表面抗体(HBsAb)酶联免疫吸附测定试剂盒
AEFI00444-02 192次 2-8C 操作手册
1 1 室温
*注意：在使用之前，应将溶液离心至管底。
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FAQ
1. RT-PCR反应后，无PCR产物或出现Smear，怎么办？
此时请从提取的RNA样品的纯度和添加量、基因的表达丰度、基因的长度和GC含量、引物的设计情况、参考文献的可信度以及RT-PCR条件的设定等方面加以考虑。
2. PCR反应时，反转录反应液的使用量使用多少较为合适？
反转录反应液的使用量可控制在1 μl～10 μl的范围。
3. 3′RACE PCR扩增产物经电泳分析后，有时为什么出现多条带现象？
可能是因为未知序列信息不清楚导致了非特异性扩增； 扩增的基因为多基因家族的成员；mRNA不同的拼接方式造成的。
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