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在大肠杆菌中生产的重组人生长激素是一条由191个氨基酸组成的单一非糖基化多肽链,分子量为22千道尔顿。该生长激素通过专属色谱技术进行纯化。
GH蛋白由含甘露醇和甘氨酸的0.2微米过滤浓缩溶液冻干制得
经SDS-聚丙烯酰胺凝胶电泳检测,纯度大于96.0%。
通过大鼠淋巴瘤NB2-11细胞增殖实验测定了半数有效剂量(ED50),结果显示其小于0.1纳克/毫升。
Introduction
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Synonyms
GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized GH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGH should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Solubility
It is recommended to reconstitute the lyophilized HGH in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F.
关键词:- 重组人生长激素
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产品组分
组分内容
型号 规格 储存温度
重组人生长激素
CYT-202-01 100ug -20°C 重组人生长激素
CYT-202-02 500ug -20°C 重组人生长激素
CYT-202-03 1mg -20°C 操作手册
1 1 室温
*注意:在使用之前,应将溶液离心至管底。
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注意事项
保存建议 推荐蓝冰或冰袋运输。当您收到产品后,按照说明书建议保存各组分。 警告 本品仅供科研使用,请勿用于临床与诊断。 -
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