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表皮生长因子受体（EGFR；ErbB-1；HER1）是表皮生长因子家族成员的细胞表面受体。EGFR 激酶的过表达和/或过度激活与多种人类癌症相关，例如肺癌、胶质母细胞瘤以及头颈部上皮肿瘤，这推动了靶向 EGFR 的抗癌治疗药物的研发。在约 40% 的 EGFR 突变型肺肿瘤中可检测到 EGFR（L858R）突变。EGFR（L858R）激酶检测试剂盒旨在以 Kinase-Glo MAX 作为检测试剂，测定 EGFR（L858R）激酶的活性，适用于筛选和分析相关应用。
Assay Kit Format
Luminescent
Species
Human
Supplied As
The EGFR (L858R) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(L858R) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubatorTarget(s)
EGFR
UniProt #
P00533
Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
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产品组分
内容 型号
规格 储存温度
EGFR(L858R)激酶检测试剂盒
40324-01 96次
-20°C EGFR(L858R)激酶检测试剂盒 40324-02 384次
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
1. Nakamura, J.L. Expert Opin. Ther. Targets 11(4):463-472 (2007)
2. Tsai, M-F., et al. Scientific Reports 5: 13574 (2015)
Date: 2026 Jun 30 Journal: ACS omega Authors: Osmaniye D, Balıkçı Ü, Kurban B, Özkay Y, Kaplancıklı ZA
Date: 2023 Apr 17 Journal: RSC advances Authors: Ismail MA, Abusaif MS, El-Gaby MSA, Ammar YA, Ragab A
Date: 2022 Jul 12 Journal: Pharmaceuticals (Basel, Switzerland) Authors: Hassanin MA, Mustafa M, Abourehab MAS, Hassan HA, Aly OM, Beshr EAM
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