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CDK2检测试剂盒

CDK2检测试剂盒,CDK2 Assay Kit,旨在以 KinaseGlo MAX 作为检测试剂,用于测定细胞周期蛋白依赖性激酶2/细胞周期蛋白A2的活性,用于筛选和活性分析相关应用。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。


关键词

CDK2检测试剂盒



目录号

79599

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  • 细胞周期蛋白依赖性激酶(CDKs)是细胞周期的关键调节因子。细胞周期蛋白依赖性激酶仅在与其调节蛋白细胞周期蛋白结合时才具有活性。为实现成功的细胞分裂,细胞周期蛋白依赖性激酶的活性受到严格调控。由于异常的细胞分裂是癌症病理的表现,因此调控细胞周期蛋白依赖性激酶的活性已被证实为一种极具前景的治疗策略。其中,细胞周期蛋白依赖性激酶2在DNA复制中发挥重要作用。该细胞周期蛋白依赖性激酶2检测试剂盒以Kinase-Glo MAX作为检测试剂,用于测定细胞周期蛋白依赖性激酶2/细胞周期蛋白A2的活性,适用于筛选和活性分析相关应用。该试剂盒采用便捷的96孔板格式,配备的纯化重组细胞周期蛋白依赖性激酶2/细胞周期蛋白A2酶、细胞周期蛋白依赖性激酶底物肽、三磷酸腺苷(ATP)及激酶检测缓冲液可满足100次酶促反应的需求。

    Assay Kit Format

    Luminescent

    Species

    Human

    Supplied As

    The CDK2 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK2/CyclinA2 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.

    Materials Required But Not Supplied

    Kinase-Glo MAX (Promega #V6071)
    Dithiothreitol (DTT, 1 M; optional)
    Microplate reader capable of reading luminescence
    Adjustable micropipettor and sterile tips
    30°C incubator

    Target(s)

    CDK2

    UniProt #

    P24941, P2024

    Stability

    Stable at least 6 months from date of receipt. when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

    Instructions for Use

    See assay kit data sheet for detailed protocol.

    Shipping Temperature

    -80°C

    Warnings

    Avoid freeze/thaw cycles.

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    型号

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    储存温度

    CDK2检测试剂盒

    79599-01

    96次

    -20°C
    CDK2检测试剂盒 79599-02

    384次

    -20°C

    操作手册

    1 1

    常温

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    79599-CDK2检测试剂盒

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  • 注意事项

    保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

     

  • FAQ

  • References

    Uzma A. et. al., Nature Review Drug Discovery 14:130-146 (2015)

    Thiazole-Derived Dual EGFR/CDK-2 Inhibitors: Rational Design, Synthesis, In Vitro Anticancer Evaluation, Mechanistic Profiling, and Computational Binding Analysis.

    Date: 2026 Jul   Journal: Archiv der Pharmazie   Authors: Nasr R, Nafie MS, Haffez H, Moustafa MAA, El-Kerdawy MM, Ali AR


    Antimicrobial, anticancer activities and molecular docking of eco-friendly chitosan nanocapsule loaded with biosynthesized titanium nanoparticles by Aspergillus flavus.

    Date: 2026   Journal: Frontiers in microbiology   Authors: El-Gazzar N, Rafat M, Rabie G, Sitohy B


    In Silico and in vitro evaluation of the anticancer effect of a 1,5-Benzodiazepin-2-One derivative (3b) revealing potent dual inhibition of HER2 and HDAC1.

    Date: 2025 Apr 18   Journal: Scientific reports   Authors: Al-Theyab NS, Bakheit AH, Lahmidi S, Alanazi MM, Ali AM, Azzaoui K, Essassi EM, Mague JT, Hefnawy M, Alanazi MM, Alanazi AS


    Design, Synthesis and Molecular Modeling of Pyrazolo[1,5-a]pyrimidine Derivatives as Dual Inhibitors of CDK2 and TRKA Kinases with Antiproliferative Activity.

    Date: 2024 Dec 10   Journal: Pharmaceuticals (Basel, Switzerland)   Authors: Attia MH, Lasheen DS, Samir N, Taher AT, Abdel-Aziz HA, Abou El Ella DA


    Novel meriolin derivatives potently inhibit cell cycle progression and transcription in leukemia and lymphoma cells via inhibition of cyclin-dependent kinases (CDKs).

    Date: 2024 Jun 11   Journal: Cell death discovery   Authors: Schmitt L, Hoppe J, Cea-Medina P, Bruch PM, Krings KS, Lechtenberg I, Drießen D, Peter C, Bhatia S, Dietrich S, Stork B, Fritz G, Gohlke H, Müller TJJ, Wesselborg S


    The role of RNF138 in DNA end resection is regulated by ubiquitylation and CDK phosphorylation.

    Date: 2024 Mar   Journal: The Journal of biological chemistry   Authors: Locke AJ, Abou Farraj R, Tran C, Zeinali E, Mashayekhi F, Ali JYH, Glover JNM, Ismail IH


    Novel tetrahydroisoquinolines as DHFR and CDK2 inhibitors: synthesis, characterization, anticancer activity and antioxidant properties.

    Date: 2024 Feb 16   Journal: BMC chemistry   Authors: Sayed EM, Bakhite EA, Hassanien R, Farhan N, Aly HF, Morsy SG, Hassan NA


    Novel benzo chromene derivatives: design, synthesis, molecular docking, cell cycle arrest, and apoptosis induction in human acute myeloid leukemia HL-60 cells.

    Date: 2023 Dec   Journal: Journal of enzyme inhibition and medicinal chemistry   Authors: Abd El-Hameed RH, Mohamed MS, Awad SM, Hassan BB, Khodair MAE, Mansour YE


    Synthesis and Evaluation of Some New 4H-Pyran Derivatives as Antioxidant, Antibacterial and Anti-HCT-116 Cells of CRC, with Molecular Docking, Antiproliferative, Apoptotic and ADME Investigations.

    Date: 2022 Jul 19   Journal: Pharmaceuticals (Basel, Switzerland)   Authors: El-Sayed NNE, Zaki MEA, Al-Hussain SA, Ben Bacha A, Berredjem M, Masand VH, Almarhoon ZM, Omar HS


    Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.

    Date: 2022 May 20   Journal: Nature communications   Authors: Zhang J, Gan Y, Li H, Yin J, He X, Lin L, Xu S, Fang Z, Kim BW, Gao L, Ding L, Zhang E, Ma X, Li J, Li L, Xu Y, Horne D, Xu R, Yu H, Gu Y, Huang W

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