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细胞周期蛋白依赖性激酶(CDKs)是细胞周期的关键调节因子。细胞周期蛋白依赖性激酶仅在与其调节蛋白细胞周期蛋白结合时才具有活性。为实现成功的细胞分裂,细胞周期蛋白依赖性激酶的活性受到严格调控。由于异常的细胞分裂是癌症病理的表现,因此调控细胞周期蛋白依赖性激酶的活性已被证实为一种极具前景的治疗策略。其中,细胞周期蛋白依赖性激酶2在DNA复制中发挥重要作用。该细胞周期蛋白依赖性激酶2检测试剂盒以Kinase-Glo MAX作为检测试剂,用于测定细胞周期蛋白依赖性激酶2/细胞周期蛋白A2的活性,适用于筛选和活性分析相关应用。该试剂盒采用便捷的96孔板格式,配备的纯化重组细胞周期蛋白依赖性激酶2/细胞周期蛋白A2酶、细胞周期蛋白依赖性激酶底物肽、三磷酸腺苷(ATP)及激酶检测缓冲液可满足100次酶促反应的需求。
Assay Kit Format
Luminescent
Species
Human
Supplied As
The CDK2 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK2/CyclinA2 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubatorTarget(s)
CDK2
UniProt #
P24941, P2024
Stability
Stable at least 6 months from date of receipt. when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
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产品组分
内容 型号
规格 储存温度
CDK2检测试剂盒
79599-01 96次
-20°C CDK2检测试剂盒 79599-02 384次
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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References
Uzma A. et. al., Nature Review Drug Discovery 14:130-146 (2015)
Date: 2026 Jul Journal: Archiv der Pharmazie Authors: Nasr R, Nafie MS, Haffez H, Moustafa MAA, El-Kerdawy MM, Ali AR
Date: 2026 Journal: Frontiers in microbiology Authors: El-Gazzar N, Rafat M, Rabie G, Sitohy B
Date: 2025 Apr 18 Journal: Scientific reports Authors: Al-Theyab NS, Bakheit AH, Lahmidi S, Alanazi MM, Ali AM, Azzaoui K, Essassi EM, Mague JT, Hefnawy M, Alanazi MM, Alanazi AS
Date: 2024 Dec 10 Journal: Pharmaceuticals (Basel, Switzerland) Authors: Attia MH, Lasheen DS, Samir N, Taher AT, Abdel-Aziz HA, Abou El Ella DA
Date: 2024 Jun 11 Journal: Cell death discovery Authors: Schmitt L, Hoppe J, Cea-Medina P, Bruch PM, Krings KS, Lechtenberg I, Drießen D, Peter C, Bhatia S, Dietrich S, Stork B, Fritz G, Gohlke H, Müller TJJ, Wesselborg S
The role of RNF138 in DNA end resection is regulated by ubiquitylation and CDK phosphorylation.
Date: 2024 Mar Journal: The Journal of biological chemistry Authors: Locke AJ, Abou Farraj R, Tran C, Zeinali E, Mashayekhi F, Ali JYH, Glover JNM, Ismail IH
Date: 2024 Feb 16 Journal: BMC chemistry Authors: Sayed EM, Bakhite EA, Hassanien R, Farhan N, Aly HF, Morsy SG, Hassan NA
Date: 2023 Dec Journal: Journal of enzyme inhibition and medicinal chemistry Authors: Abd El-Hameed RH, Mohamed MS, Awad SM, Hassan BB, Khodair MAE, Mansour YE
Date: 2022 Jul 19 Journal: Pharmaceuticals (Basel, Switzerland) Authors: El-Sayed NNE, Zaki MEA, Al-Hussain SA, Ben Bacha A, Berredjem M, Masand VH, Almarhoon ZM, Omar HS
Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.
Date: 2022 May 20 Journal: Nature communications Authors: Zhang J, Gan Y, Li H, Yin J, He X, Lin L, Xu S, Fang Z, Kim BW, Gao L, Ding L, Zhang E, Ma X, Li J, Li L, Xu Y, Horne D, Xu R, Yu H, Gu Y, Huang W
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