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与细胞周期蛋白H和MAT1组装的细胞周期蛋白依赖性激酶7（CDK7）包括转录因子TFIIH的重要子组件。众所周知，它具有双重作用，包括通过磷酸化RNA聚合酶II的C末端结构域（CTD）进行转录调节，以及作为CDK激活激酶（CAK）控制细胞周期进展。CDK7检测试剂盒旨在测量CDK7/Cyclin H/MAT1活性，用于筛选和分析应用，使用ADP-Glo®作为检测试剂。
Assay Kit Format
Luminescent
Species
Human
Supplied As
The CDK7 Assay Kit comes in a convenient 96-well format, with enough purified recombinant CDK7/Cyclin H/MAT1 enzyme, CDK substrate peptide, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
ADP-Glo® Kinase Assay (Promega #V6930)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubatorTarget(s)
CDK7
UniProt #
Q24216
Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
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产品组分
内容 型号
规格 储存温度
CDK7检测试剂盒
79603-01 96次
-20°C CDK7检测试剂盒 79603-02 384次
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
Uzma A. et al., Nature Review Drug Discovery 14:130-146 (2015)
Date: 2025 Jun 4 Journal: Molecular cancer therapeutics Authors: Xie X, Manai M, Rampa DR, Fuson JA, Nakasone ES, Pearson T, Kuntal BS, Tripathy D, Ueno NT, Lee J
CDK7/CDK9 mediates transcriptional activation to prime paraptosis in cancer cells.
Date: 2024 Jun 10 Journal: Cell & bioscience Authors: Chiang SK, Chang WC, Chen SE, Chang LC
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