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人源TRKC，亦称为NTKR3、神经营养性酪氨酸激酶受体3型及NT-3受体，GenBank登录号为NM_002530，氨基酸位点456-825（末端），带有N端谷胱甘肽S-转移酶标签，通过杆状病毒表达系统在草地贪夜蛾9细胞中表达，分子量为69千道尔顿。
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity
≥38%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
MW
69 kDa
Amino Acids
456–825(end)
Specific Activity
1.0 pmol/min/μg
Genbank #
NM_002530.4
UniProt #
Q16288
Tag(s)
N-terminal GST-tag
Stability
>6 months at –80°C.
Assay Conditions
Activity determined using a DELFIA-assay with a poly-(Glu:Tyr 4:1)-biotin-coated plate, for 30 min. at room temperature.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles
Scientific Category
Neuroscience, Tyrosine Kinase
Regulatory Status
Research Use Only
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产品组分
内容 型号
规格 储存温度
GST标签重组TRKC蛋白 40282-01 10ug -20°C GST标签重组TRKC蛋白 40282-02 20ug -20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
1. Lamballe, F., et al. (1991). Cell 66 (5): 967–979.
2. Tessarollo, L., et al. (1993). Development 118 (2): 463–75.
3. McGregor, L.M., et al. (1994). Genomics 22 (2): 267–72.Date: 2024 Dec 2 Journal: Cancer discovery Authors: Lin JJ, Horan JC, Tangpeerachaikul A, Swalduz A, Valdivia A, Johnson ML, Besse B, Camidge DR, Fujino T, Yoda S, Nguyen-Phuong L, Mizuta H, Bigot L, Nobre C, Lee JB, Yu MR, Mente S, Sun Y, Kohl NE, Porter JR, Shair MD, Zhu VW, Felip E, Cho BC, Friboulet L, Hata AN, Pelish HE, Drilon A
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