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HER4（人表皮生长因子受体4），也被称为ErbB4或受体酪氨酸激酶4，是EGFR家族的一种受体酪氨酸激酶。它是心脏、神经系统和乳腺发育所必需的物质，也存在于神经肌肉接头的突触后侧和表皮中。HER4可被神经调节蛋白2-4、β细胞素以及肝素结合性表皮生长因子样生长因子（HB-EGF）激活，并参与细胞有丝分裂和细胞分化过程。HER4与配体结合后会发生构象变化，与其他HER蛋白形成同二聚体或异二聚体，进而激活丝裂原活化蛋白激酶（MAPK）通路和磷脂酰肌醇3-激酶（PI3K）/AKT通路。该蛋白的突变可能引发癌症，同时也与19型肌萎缩侧索硬化症（ALS）和精神分裂症相关。该蛋白作为癌基因或抑癌基因的作用尚未完全明确，可能取决于癌症类型。目前已开发出多种HER4抑制剂并正在开展临床试验。阿法替尼是一种泛HER抑制剂，已获批用于治疗非小细胞肺癌（NSCLC），但可能导致急性肝损伤甚至死亡。研发针对HER4的高特异性抑制剂有望在肿瘤学领域发挥重要作用。
重组人HER4（人表皮生长因子受体4，又称ErbB4），包含26-651位氨基酸（胞外结构域）。该构建体在C端融合了人IgG1的Fc结构域，其后连接一个C端Avi-Tag™标签。该蛋白经亲和纯化获得。
Species
Human
Construct
HER4 (ErbB4) (26-651-Fc(lgG1)-Avi)
Host Species/Expression System
HEK293
Purity
≥90%
Format
Aqueous buffer solution.
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Target(s)
HER4
MW
99 kDa + glycans
Amino Acids
26-651
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Genbank #
NM_005235.3
UniProt #
Q15303
Tag(s)
C-terminal Fc-Avi-Tag
Stability
At least 6 months at -80°C.
Assay Conditions
The NRG1β Protein, Human (HEK293) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 0.5 µg/ml in PBS). The plate was washed 3 times with PBST and blocked using 200 µl of Blocking Buffer 3 for 2 hours at Room Temperature (RT). After removing the blocking buffer, 50 µl/well of purified HER4, serially diluted in 1x PP-02 Buffer, was added for 1 hour at RT. After washing, the plate was incubated for 40 minutes with HRP-labeled anti-FC antibody diluted 1:1000 in Blocking Buffer 3 (50 µl/well) and washed again. Finally, 100 µl of ECL substrate was added to each well and plate was read immediately in a luminometer or microtiter-plate reader capable of reading chemiluminescence.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for studying the binding of HER4 in ELISA and in cellular assays.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
Scientific Category
Kinase
Regulatory Status
Research Use Only
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产品组分
内容 型号
规格 储存温度
Avi-Tag重组HER4(ErbB4)Fc融合蛋白 102238-01 25ug -20°C Avi-Tag重组HER4(ErbB4)Fc融合蛋白 102238-02 100ug -20°C Avi-Tag重组HER4(ErbB4)Fc融合蛋白 102238-03 1mg -20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
1. Lamballe, F., et al. (1991). Cell 66 (5): 967–979.
2. Tessarollo, L., et al. (1993). Development 118 (2): 463–75.
3. McGregor, L.M., et al. (1994). Genomics 22 (2): 267–72.Date: 2024 Dec 2 Journal: Cancer discovery Authors: Lin JJ, Horan JC, Tangpeerachaikul A, Swalduz A, Valdivia A, Johnson ML, Besse B, Camidge DR, Fujino T, Yoda S, Nguyen-Phuong L, Mizuta H, Bigot L, Nobre C, Lee JB, Yu MR, Mente S, Sun Y, Kohl NE, Porter JR, Shair MD, Zhu VW, Felip E, Cho BC, Friboulet L, Hata AN, Pelish HE, Drilon A
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