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精氨酸酶通过水解作用将精氨酸转化为鸟氨酸。精氨酸酶存在两种已知的同工型，即ARG1和ARG2。该酶参与多种免疫反应的调控，是免疫治疗的主要靶点。ARG1/2在髓源性抑制细胞（MDSCs）和肿瘤相关巨噬细胞（TAMs）中过表达。ARG1/2的过表达会导致细胞内和细胞外的精氨酸水平耗尽。当微环境中的精氨酸被耗尽时，免疫细胞会缺乏这种氨基酸，关键免疫激活因子的功能也会受损；T细胞增殖受到抑制，调节性T细胞被激活并抑制CD4+ T细胞，同时免疫抑制剂的存活时间延长。精氨酸的耗尽还会导致肿瘤相关巨噬细胞和髓源性抑制细胞释放活性氮物质和活性氧物质。这些活性物质会引发T细胞凋亡，并促进抗原呈递细胞的活化与增殖。
ARG1抑制剂筛选检测试剂盒专为筛选和表征应用而检测 ARG1（精氨酸酶 1）的活性。该检测试剂盒采用便捷的 96 孔或 384 孔板格式，配备足量的纯化重组 ARG1（氨基酸 1-322）、硫代精氨酸底物、检测缓冲液和检测试剂，可进行 100 或 400 次酶促反应。
Assay Kit Format
Colorimetric - UV Spectrophotometry
Species
Human
Supplied As
The assay kit comes in a convenient 96-well or 384-well format, with enough purified recombinant ARG1 (amino acids 1-322), thioarginine substrate, assay buffer and detection reagent for 96 or 384 enzyme reactions.
Materials Required But Not Supplied
- Spectrophotometer capable of measuring absorbance at λ= 410–415 nm.
- Ethanol (200 proof)
Target(s)
ARG1
UniProt #
P05089
Stability
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Study enzyme kinetics and screen small molecule inhibitors for drug discovery in high throughput screening (HTS) applications
Shipping Temperature
-80°C
Notes
Contraindications
• The final concentration of DMSO in the assay should not exceed 1%.
• The assay should not be performed in the presence of strong acids or bases, ionic detergents, and high salt.
• Compounds that are fluorescent may interfere with the results, depending on their spectral excitation and emission properties.
• It is recommended that the compound alone be tested to determine any potential interference of the compound with the assay results.Warnings
Avoid freeze/thaw cycles.
Scientific Category
Immunotherapy
Regulatory Status
Research Use Only
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产品组分
内容 型号
规格 储存温度
ARG1抑制剂筛选检测试剂盒
72048-01 96次
-20°C ARG1抑制剂筛选检测试剂盒 72048-02 384次
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
Sedbrook J.C., et al., 1999 PNAS 96(3):1140-1145.
Woll P.J., et al., 1998 PNAS 85(6):1859-1863.
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