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初始T细胞的活化需要两个信号，一是T细胞受体特异性识别抗原呈递细胞（APC）表面的MHC/抗原，二是抗原呈递细胞上的B7-1（CD80）配体与T细胞表面的CD28受体结合。CD28与B7-1的相互作用是调控参与自身免疫、炎症、肿瘤识别以及免疫耐受的T细胞的重要药物靶点。
CD28:B7-1[生物素标记]抑制剂筛选检测试剂盒专为筛选和分析CD28:B7-1信号通路的抑制剂而设计。该试剂盒的核心在于利用链霉亲和素-辣根过氧化物酶（HRP）高灵敏检测生物素标记的B7-1。本检测仅需在酶标板上完成数个简单步骤：首先，将CD28包被于96孔板；随后，使B7-1与板上的CD28共孵育；最后，用链霉亲和素-HRP处理板孔，再加入HRP底物以产生化学发光信号，通过化学发光检测仪即可对其进行测定。
Assay Kit Format
Chemiluminescent
Species
Human
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CD28, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied
PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Rotating or rocker platformTarget(s)
B7-1
UniProt #
P10747
Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol
Applications
This kit is useful for screening for inhibitors of B7-1 binding to CD28.
Shipping Temperature
-80°C (dry ice)
Contraindications
DMSO >1%, strong acids or bases, ionic detergents, high salt.
Warnings
Avoid freeze/thaw cycles.
Scientific Category
Cell Surface Receptor/Single-pass Transmembrane Protein.
Regulatory Status
Research Use Only
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产品组分
内容 型号
规格 储存温度
CD28:B7-1[生物素化]抑制剂筛选检测试剂盒
72007-01 96次
-20°C CD28:B7-1[生物素化]抑制剂筛选检测试剂盒 72007-02 384次
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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References
1. Keir, M.E., et al. Immunol. Rev. 2005, 204: 128-143.
2. Haas, C., et al. Int. J. Cancer. 2006, 1;118: 658-667.AI-Designed Cyclic Peptides Enable Controllable Modulation of the CD28 Immune Checkpoint.
Date: 2026 May 30 Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany) Authors: Kuncewicz K, Upadhyay S, Zhu R, Duan H, Gabr MT
Date: 2025 Dec 11 Journal: Journal of medicinal chemistry Authors: Upadhyay S, Cho S, Nada H, Gabr MT
Date: 2026 Jan Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie Authors: Upadhyay S, Nada H, Cho S, Gabr MT
Date: 2025 Dec 15 Journal: European journal of medicinal chemistry Authors: Upadhyay S, Talagayev V, Cho S, Wolber G, Gabr M
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