- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
PD-1受体与PD-L1配体结合后发出的细胞信号会减弱免疫反应，且肿瘤和病毒都会利用这一机制。PD-1:PDL1[生物素化]抑制剂筛选比色法检测试剂盒旨在筛选能抑制PD-L1配体与PD-1受体结合的抑制剂。该试剂盒的关键在于生物素标记的PD-L1与链霉亲和素-辣根过氧化物酶（HRP）之间的高亲和力。该检测仅需在酶标板上进行几个简单步骤。首先，将PD-1包被在96孔板上；随后，使PD-L1与板上的PD-1共孵育；最后，用链霉亲和素-HRP处理板，再加入比色法HRP底物以产生颜色，随后可使用紫外-可见分光光度计酶标仪对颜色进行检测。
Assay Kit Format
Colorimetric
Species
Human
Supplied As
This kit comes in a convenient 96-well format, with biotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied
PBS (Phosphate buffered saline)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used, but sensitivity of the assay will be
greatly reduced.Target(s)
PD-1
UniProt #
Q15116, Q9NZQ7
Stability
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Instructions for Use
See assay kit data sheet for detailed protocol
Applications
This kit is useful for screening for inhibitors of PD-1 binding to PD-L1.
Shipping Temperature
-80°C (dry ice)
Warnings
Avoid freeze/thaw cycles.
Scientific Category
Immunotherapy & Cell Surface Receptors
Regulatory Status
Research Use Only
-
产品组分
内容 型号
规格 储存温度
PD-1:PD-L1[生物素化]抑制剂筛选比色检测试剂盒
72016-01 96次
-20°C PD-1:PD-L1[生物素化]抑制剂筛选比色检测试剂盒 72016-02 384次
-20°C 操作手册
1 1 常温
-
营销中心
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
-
References
1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.Application Reference(s):
1. Selective Suppression of Cell Growth and Programmed Cell Death-Ligand 1 Expression in HT1080 Fibrosarcoma Cells by Low Molecular Weight Fucoidan Extract (2019)
在线留言
如果您对我们的产品感兴趣，请留下您的信息，我们将尽快与您联系，谢谢！
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们
