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肝脏型谷氨酰胺酶(GLS2)是一种磷酸激活的酰胺水解酶,可催化L-谷氨酰胺水解为L-谷氨酸和氨。GLS2与肿瘤进展及癌症相关,包括乳腺癌和肝细胞癌。
肝脏型谷氨酰胺酶(GLS2)抑制剂筛选检测试剂盒旨在用于检测 GLS2 的水解酶活性,以满足筛选和特征分析的应用需求。
Assay Kit Format
Fluorogenic
Species
Human
Supplied As
The GLS2 assay kit comes in a convenient 96-well format, with purified GLS2, its substrates, the Coupling reagent, and buffer for 100 enzyme reactions.
Materials Required But Not Supplied
0.5 M dithiothreitol (DTT) in aqueous solution
Adjustable micropipettor and sterile tips
Fluorescent microplate reader capable of reading λexcitation = 340 nm; λemission = 460 nmUniProt #
Q9UI32
Stability
Up to 6 months from date of receipt, when stored as recommended.
Instructions for Use
See assay protocol for detailed instructions
Shipping Temperature
-80°C
Scientific Category
Metabolism
Regulatory Status
Research Use Only
关键词:- 肝脏型谷氨酰胺酶(GLS2)抑制剂筛选检测试剂盒
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产品组分
内容 型号
规格 储存温度
肝脏型谷氨酰胺酶(GLS2)抑制剂筛选检测试剂盒
79925-01 96次
-20°C 肝脏型谷氨酰胺酶(GLS2)抑制剂筛选检测试剂盒 79925-02 384次
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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References
- Xiang, Lisha, et al. (2013) "Knock-down of glutaminase 2 expression decreases glutathione, NADH, and sensitizes cervical cancer to ionizing radiation." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1833.12: 2996-3005.
- Zhang, Jianbin, et al. (2013) "Epigenetic silencing of glutaminase 2 in human liver and colon cancers." BMC cancer 13.1: 601-609.
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