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CD2是一种跨膜糖蛋白,也被称为LFA-2,作为黏附分子和共刺激受体发挥作用,在T细胞活化、自然杀伤细胞毒性以及免疫突触形成中起到关键作用。它与抗原呈递细胞上的CD58(LFA-3)结合,以促进T细胞与抗原呈递细胞(APC)的相互作用。CD2是胸腺内T细胞早期发育过程中表达的最早标志物之一,并在整个T细胞成熟过程中持续表达。它被视为全T细胞标志物,使其成为大多数T细胞谱系的可靠标志物。CD2也在大多数自然杀伤(NK)细胞以及一部分自然杀伤T(NKT)细胞上表达。在来自健康个体的外周血单个核细胞(PBMCs)中,CD2阳性细胞通常约占总细胞群体的60%-90%。
人CD2阳性分离试剂盒旨在通过磁性从复杂的免疫细胞群中分离表达CD2的细胞。该试剂盒经过优化,可从正常人外周血单个核细胞(PBMCs)中快速分离/去除CD2阳性细胞。在加入SpeediSort™ 分离磁珠前,先将细胞与CD2靶向抗体孵育,随后置于磁铁上实现快速简便的分离。置于磁铁上时,CD2阳性细胞会固定在试管侧壁,而CD2阴性细胞则保持悬浮状态,从而实现便捷分离。
Species
Human
Supplied As
This kit is provided with enough reagents and materials for isolation or depletion of CD2+ cells from up to 1x108 or 1x109 PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC amounts.
UniProt #
P06729
Stability
This isolation kit will perform optimally for up to 6 months from the date of receipt when the materials are stored as directed.
Applications
- Isolate CD2-expressing cells from mixed populations such as PBMCs, whole blood, or tissue-derived lymphocyte suspensions to enrich for total T cells and NK cells while excluding B cells and monocytes.
- CD2+ cells may be used for downstream applications such as genomic analysis, expression assays, immune repertoire analysis, protein isolation, flow cytometry, or functional studies such as cytotoxicity assays.
Shipping Temperature
-20°C
Warnings
Avoid freeze/thaw cycles.
Safety
This product is for research purposes only and is not intended for human or therapeutic use. This product contains small amounts of sodium azide. This product should be considered hazardous and harmful by inhalation, in contact with skin, eyes, clothing, and if swallowed. If contact occurs, wash thoroughly.
Scientific Category
Immune Cell Markers (ICM), Immune Cell Signaling (ICSig)
Regulatory Status
Research Use Only
关键词:- 人CD2阳性分离试剂盒
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产品组分
内容 型号
规格 储存温度
人CD2阳性分离试剂盒
82180-01 1x108
-20°C 人CD2阳性分离试剂盒
82180-02 1x109
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
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