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6-硫代-GTP

6-硫代-GTP,6-Thio-GTP,6-Thio-guanosine-5'-triphosphate, Sodium salt,6-硫代鸟苷-5'-三磷酸,钠盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。


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6-硫代-GTP


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目录号

NU1106

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  • For general laboratory use.

    Please centrifuge briefly before opening (volume ≤2 ml).

    Shipping: shipped on gel packs

    Storage Conditions: store at -20 °C
    Short term exposure (up to 1 week cumulative) to ambient temperature possible.

    Shelf Life: 12 months after date of delivery

    Molecular Formula: C10H16N5O13P3S (free acid)

    Molecular Weight: 539.24 g/mol (free acid)

    Exact Mass: 538.97 g/mol (free acid)

    CAS#: 17670-19-8

    Purity: ≥ 95 % (HPLC)

    Form: solution in water

    Concentration: 10 mM - 11 mM

    pH: 7.5 ±0.5

    Spectroscopic Properties: λmax 257 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5), λmax 342 nm, ε 24.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)

    Applications:
    Allosteric effect on E.coli CTP-synthase[1]
    Inhibition of Vac-1-Rac signalling[2]
    Immunosuppression by blockade of GTPase activation[3, 4]
    Immunosuppressive drug (transplantation, inflammation)[1, 4]

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    型号

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    6-硫代-GTP

    NU1106-01

    150ul(10mM)

    -20°C

    6-硫代-GTP

    NU1106-02

    750ul(10mM)

    -20°C

    操作手册

    1 1

    常温

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    NU1106-6-硫代-GTP

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    NU1106-6-硫代-GTP相关单页

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  • 注意事项

    保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

     

  • FAQ

     

  • Selected References:
    [1] Lunn et al. (2008) Structural requirements for the activation of Escherichia coli CTP synthase by the allosteric effector GTP are stringent, but requirements for inhibition are lax. J. Biol Chem. 283:2010.
    [2] Wang et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signaling blockade in a mouse model. Transplant Immunology 18:53.
    [3] Poppe et al. (2005) Azothioprine suppresses Ezrin-Radixin-Moesin-dependent T cell-APC conjugation through inhibition of Vav Guanosine exchange activity on Rac proteins. J. Immunology 176:640.
    [4] Tiede et al. (2003) CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4+t lymphocytes. J. Clinical Investigation 111:1133.
    Karner et al. (2010) Determination of 6-thioguanosine diphosphate and triphosphate and nucleoside diphosphate kinase activity in erythrocytes: novel targets for thiopurine therapy? Ther. Drug Monit. 32 (2):119.
    Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.
    Du et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signalling blockade in a mouse model. Transplant Immunology 18 (1): 53.
    Pestova et al. (2006) Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex. Genes & Development 20:624.
    Neurath et al. (2005) 6-Thioguanosine Diphosphate and Triphosphate Levels in Red Blood Cells and Response to Azathioprine Therapy in Crohn's Disease. Clinical Gastroenterology and Hepatology 3 (10): 1007.
    Poland et al. (1997) Entrapment of 6-thiophosphoryl-IMP in the active site of crystalline adenylosuccinate synthetase from Escherichia coli. J. Biol. Chem. 272 (24):15200.
    Yarbrough et al. (1985) Kinetics of interaction of 2-amino-6-mercapto-9-beta-ribofuranosylpurine 5'-triphosphate with bovine brain tubulin. Biochemistry-US 24 (7):1708.
    Fishback et al. (1984) Interaction of 6-mercapto-GTP with bovine brain tubulin - equilibrium aspects. J. Biol. Chem. 259 (3):1968.

    SERS spectroscopy as a tool for the study of thiopurine drug pharmacokinetics in a model of human B leukemia cells.

    Sofia Pagarin, Anna Bolognese, ..., Alois Bonifacio

    Chemico-biological interactions  |  2023 Dec 25  |  37944627

    Rac1/pSTAT3 expression: A pharmacodynamic marker panel as a first step toward optimization of thiopurine therapy in inflammatory bowel disease patients.

    Debbie S Deben, Arjan J van Adrichem, ..., Mathie P G Leers

    Cytometry. Part A : the journal of the Int…  |  2022 May 02  |  34595833

    Development of a Simple In Vitro Assay To Identify and Evaluate Nucleotide Analogs against SARS-CoV-2 RNA-Dependent RNA Polymerase

    Gaofei Lu, Xi Zhang, ..., Wen Xiong

    Antimicrobial Agents and Chemotherapy  |  2020 Dec 16  

    A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand.

    Stephanie Jung, Tina von Thülen, ..., Stefan Bauer

    Nucleic Acids Research  |  2020 Nov 04  |  32946572

    Discovery of five HIV nucleoside analog reverse-transcriptase inhibitors (NRTIs) as potent inhibitors against the RNA-dependent RNA polymerase (RdRp) of SARS-CoV and 2019-nCoV

    Sun Jialei

    bioRxiv  |  2020 Nov 01 

    Milliscale Self-Integration of Megamolecule Biopolymers on a Drying Gas-Aqueous Liquid Crystalline Interface.

    Kosuke Okeyoshi, Maiko K Okajima, Tatsuo Kaneko

    Biomacromolecules  |  2017 Oct 10  |  27077450

    The Role of Rho-GTPases and actin polymerization during Macrophage Tunneling Nanotube Biogenesis

    Samer J. Hanna, Kessler McCoy-Simandle, ..., Dianne Cox

    Scientific Reports  |  2017 Aug 17  |  28819224

    Designer Thiopurine-analogues for Optimised Immunosuppression in Inflammatory Bowel Diseases.

    Imke Atreya, Alexandra Diall, ..., Markus F Neurath

    Journal of Crohn's and Colitis  |  2017 May 12  |  27112707

    The synaptic recruitment of lipid rafts is dependent on CD19-PI3K module and cytoskeleton remodeling molecules

    Liling Xu, Arturs Auzins, ..., Wanli Liu

    Journal of leukocyte biology  |  2015 May 15 

    Macrophage Motility is Driven by Frontal-Towing with a Force Magnitude Dependent on Substrate Stiffness

    Laurel E. Hind, Micah Dembo, Daniel A. Hammer

    Integrative biology : quantitative bioscie…  |  2015 Apr 01  |  25768202

    Vav1 regulates MHCII expression in murine resting and activated B cells.

    Bettina Jux, Andrea Staratschek-Jox, ..., Waldemar Kolanus

    International immunology  |  2014 Oct 24  |  23391492 

      Supplementary   Harmful Effects of the Azathioprine Metabolite 6-Mercaptopurine in Vascular Cells: Induction of Mineralization

    Jasmin Prüfer, Mirjam Schuchardt, ..., Yin Tintut

    PLoS ONE  |  2014 Jul 16  |  25029363

     

    T cell receptor-driven transendothelial migration of human effector memory CD4 T cells involves Vav, Rac and Myosin IIA

    Thomas D. Manes, Jordan S. Pober

    Journal of Immunology (Baltimore, Md. : 19…  |  2014 Apr 01  |  23420881

    Oxidized LDL/CD36 interaction induces loss of cell polarity and inhibits macrophage locomotion

    Young Mi Park, Judith A. Drazba, ..., Roy L. Silverstein

    Molecular Biology of the Cell  |  2012 Aug 15  

    Impaired TH2 response in patients with Vav1-deficient common variable immunodeficiency with T-cell defects.

    Nagaja Capitani, Amedeo Amedei, ..., Mario M D'Elios

    The Journal of allergy and clinical immuno…  |  2010 Sep 28  |  20638113

    Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent ωG binding to a group I intron

    Penghui Bao, Qi-Jia Wu, ..., Yi Zhang

    Nucleic Acids Research  |  2008 Dec 01  |  18978026

    Azathioprine Suppresses Ezrin-Radixin-Moesin-Dependent T Cell-APC Conjugation through Inhibition of Vav Guanosine Exchange Activity on Rac Proteins

    Daniela Poppe, Imke Tiede, ..., Markus F. Neurath

    pmc01965586  |  2007 Sep 06  |  16365460

    Enhanced cardiac allograft survival by Vav1-Rac signaling blockade in a mouse model.

    Shuang Wang, Hong Diao, ..., Caigan Du

    Transplant immunology  |  2007 Aug 09  |  17584603

    Specific functional interactions of nucleotides at key − 3 and + 4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex

    Andrey V. Pisarev, Victoria G. Kolupaeva, ..., Tatyana V. Pestova

    pmc01410799  |  2006 Mar 01  |  16510876

    CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4 + T lymphocytes

    Imke Tiede, Gerhard Fritz, ..., Markus F. Neurath

    pmc00152932  |  2003 Apr 15  |  12697733

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