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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O13P3S (free acid)
Molecular Weight: 539.24 g/mol (free acid)
Exact Mass: 538.97 g/mol (free acid)
CAS#: 17670-19-8
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 257 nm, ε 8.8 L mmol-1 cm-1 (Tris-HCl pH 7.5), λmax 342 nm, ε 24.8 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Allosteric effect on E.coli CTP-synthase[1]
Inhibition of Vac-1-Rac signalling[2]
Immunosuppression by blockade of GTPase activation[3, 4]
Immunosuppressive drug (transplantation, inflammation)[1, 4] -
产品组分
内容
型号
规格 储存温度
6-硫代-GTP
NU1106-01 150ul(10mM)
-20°C 6-硫代-GTP
NU1106-02 750ul(10mM)
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Lunn et al. (2008) Structural requirements for the activation of Escherichia coli CTP synthase by the allosteric effector GTP are stringent, but requirements for inhibition are lax. J. Biol Chem. 283:2010.
[2] Wang et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signaling blockade in a mouse model. Transplant Immunology 18:53.
[3] Poppe et al. (2005) Azothioprine suppresses Ezrin-Radixin-Moesin-dependent T cell-APC conjugation through inhibition of Vav Guanosine exchange activity on Rac proteins. J. Immunology 176:640.
[4] Tiede et al. (2003) CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4+t lymphocytes. J. Clinical Investigation 111:1133.
Karner et al. (2010) Determination of 6-thioguanosine diphosphate and triphosphate and nucleoside diphosphate kinase activity in erythrocytes: novel targets for thiopurine therapy? Ther. Drug Monit. 32 (2):119.
Bao et al. (2008) Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent wG binding to a group I intron. Nucleic Acids Research 36 (21):6934.
Du et al. (2007) Enhanced cardiac allograft survival by Vav1-Rac signalling blockade in a mouse model. Transplant Immunology 18 (1): 53.
Pestova et al. (2006) Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex. Genes & Development 20:624.
Neurath et al. (2005) 6-Thioguanosine Diphosphate and Triphosphate Levels in Red Blood Cells and Response to Azathioprine Therapy in Crohn's Disease. Clinical Gastroenterology and Hepatology 3 (10): 1007.
Poland et al. (1997) Entrapment of 6-thiophosphoryl-IMP in the active site of crystalline adenylosuccinate synthetase from Escherichia coli. J. Biol. Chem. 272 (24):15200.
Yarbrough et al. (1985) Kinetics of interaction of 2-amino-6-mercapto-9-beta-ribofuranosylpurine 5'-triphosphate with bovine brain tubulin. Biochemistry-US 24 (7):1708.
Fishback et al. (1984) Interaction of 6-mercapto-GTP with bovine brain tubulin - equilibrium aspects. J. Biol. Chem. 259 (3):1968.SERS spectroscopy as a tool for the study of thiopurine drug pharmacokinetics in a model of human B leukemia cells.
Sofia Pagarin, Anna Bolognese, ..., Alois Bonifacio
Chemico-biological interactions | 2023 Dec 25 | 37944627
Rac1/pSTAT3 expression: A pharmacodynamic marker panel as a first step toward optimization of thiopurine therapy in inflammatory bowel disease patients.
Debbie S Deben, Arjan J van Adrichem, ..., Mathie P G Leers
Cytometry. Part A : the journal of the Int… | 2022 May 02 | 34595833
Development of a Simple In Vitro Assay To Identify and Evaluate Nucleotide Analogs against SARS-CoV-2 RNA-Dependent RNA Polymerase
Gaofei Lu, Xi Zhang, ..., Wen Xiong
Antimicrobial Agents and Chemotherapy | 2020 Dec 16
A ribosomal RNA fragment with 2',3'-cyclic phosphate and GTP-binding activity acts as RIG-I ligand.
Stephanie Jung, Tina von Thülen, ..., Stefan Bauer
Nucleic Acids Research | 2020 Nov 04 | 32946572
Discovery of five HIV nucleoside analog reverse-transcriptase inhibitors (NRTIs) as potent inhibitors against the RNA-dependent RNA polymerase (RdRp) of SARS-CoV and 2019-nCoV
Sun Jialei
bioRxiv | 2020 Nov 01
Milliscale Self-Integration of Megamolecule Biopolymers on a Drying Gas-Aqueous Liquid Crystalline Interface.
Kosuke Okeyoshi, Maiko K Okajima, Tatsuo Kaneko
Biomacromolecules | 2017 Oct 10 | 27077450
The Role of Rho-GTPases and actin polymerization during Macrophage Tunneling Nanotube Biogenesis
Samer J. Hanna, Kessler McCoy-Simandle, ..., Dianne Cox
Scientific Reports | 2017 Aug 17 | 28819224
Designer Thiopurine-analogues for Optimised Immunosuppression in Inflammatory Bowel Diseases.
Imke Atreya, Alexandra Diall, ..., Markus F Neurath
Journal of Crohn's and Colitis | 2017 May 12 | 27112707
The synaptic recruitment of lipid rafts is dependent on CD19-PI3K module and cytoskeleton remodeling molecules
Liling Xu, Arturs Auzins, ..., Wanli Liu
Journal of leukocyte biology | 2015 May 15
Macrophage Motility is Driven by Frontal-Towing with a Force Magnitude Dependent on Substrate Stiffness
Laurel E. Hind, Micah Dembo, Daniel A. Hammer
Integrative biology : quantitative bioscie… | 2015 Apr 01 | 25768202
Vav1 regulates MHCII expression in murine resting and activated B cells.
Bettina Jux, Andrea Staratschek-Jox, ..., Waldemar Kolanus
International immunology | 2014 Oct 24 | 23391492
Supplementary Harmful Effects of the Azathioprine Metabolite 6-Mercaptopurine in Vascular Cells: Induction of Mineralization
Jasmin Prüfer, Mirjam Schuchardt, ..., Yin Tintut
PLoS ONE | 2014 Jul 16 | 25029363
T cell receptor-driven transendothelial migration of human effector memory CD4 T cells involves Vav, Rac and Myosin IIA
Thomas D. Manes, Jordan S. Pober
Journal of Immunology (Baltimore, Md. : 19… | 2014 Apr 01 | 23420881
Oxidized LDL/CD36 interaction induces loss of cell polarity and inhibits macrophage locomotion
Young Mi Park, Judith A. Drazba, ..., Roy L. Silverstein
Molecular Biology of the Cell | 2012 Aug 15
Impaired TH2 response in patients with Vav1-deficient common variable immunodeficiency with T-cell defects.
Nagaja Capitani, Amedeo Amedei, ..., Mario M D'Elios
The Journal of allergy and clinical immuno… | 2010 Sep 28 | 20638113
Coordination of two sequential ester-transfer reactions: exogenous guanosine binding promotes the subsequent ωG binding to a group I intron
Penghui Bao, Qi-Jia Wu, ..., Yi Zhang
Nucleic Acids Research | 2008 Dec 01 | 18978026
Azathioprine Suppresses Ezrin-Radixin-Moesin-Dependent T Cell-APC Conjugation through Inhibition of Vav Guanosine Exchange Activity on Rac Proteins
Daniela Poppe, Imke Tiede, ..., Markus F. Neurath
pmc01965586 | 2007 Sep 06 | 16365460
Enhanced cardiac allograft survival by Vav1-Rac signaling blockade in a mouse model.
Shuang Wang, Hong Diao, ..., Caigan Du
Transplant immunology | 2007 Aug 09 | 17584603
Specific functional interactions of nucleotides at key − 3 and + 4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex
Andrey V. Pisarev, Victoria G. Kolupaeva, ..., Tatyana V. Pestova
pmc01410799 | 2006 Mar 01 | 16510876
CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4 + T lymphocytes
Imke Tiede, Gerhard Fritz, ..., Markus F. Neurath
pmc00152932 | 2003 Apr 15 | 12697733
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