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Transfectamine 5000转染试剂是美国AAT生产的转染试剂,Transfectamine 5000转染试剂是一种功能强大且用途广泛的转染试剂,可将核酸引入真核细胞,引入动物细胞。它可以有效地将各种有效载荷转染到各种贴壁和悬浮细胞系中。它可用于质粒DNA转染以及基于siRNA和shRNA的基因敲低实验和基因表达研究。它对所有转染细胞均提供卓越的转染效率。Transfectamine 5000的低毒性也使转染细胞具有更高的生存能力。与大多数其他转染试剂相比,Transfectamine 5000更易于使用,并且不需要特殊的介质。IVDSHOW是AAT的中国代理商,为您提供优质的Transfectamine 5000转染试剂。
样品实验方案
简要概述
1.准备细胞进行转染
2.准备Transfectamine 5000-DNA混合物
3.将Transfectamine 5000-DNA混合物添加到细胞培养物中
4.过夜培养
5.用适当的方法分析转染效率
溶液制备
1.工作溶液配制
1.1将2.5ug DNA与200uL无血清培养基混合。
1.2在步骤1中添加7.5 uL Transfectamine 5000。
1.3充分混合并在室温下孵育20分钟。 注意:Transfectamine 5000和DNA的比例需要针对不同的细胞系进行优化,通常:Transfectamine 5000转染试剂(uL)与DNA(ug)的比例= 3-5 uL至1ug。
样品示例及操作
1.细胞培养准备
1.1转染时将细胞培养至约90%融合。
1.2转染前用新鲜的生长培养基替换。 例如,对于6孔板,每孔用2 mL培养基替换,对于10 cm板,用6 mL培养基替换。
2.转染方案
将Transfectamine 5000 -DNA混合物添加至培养板并培养过夜。 注意:重组蛋白最早可在转染后16小时开始检测。转染后72〜96小时可观察到最大表达水平。

图1.使用Transfectamine 5000,Lipofectamine 2000和Lipofectamine 3000试剂在HeLa细胞中的转染效率比较。 每种试剂用于以96孔板转染HeLa细胞,转染后24小时分析GFP表达。 与Lipofectamine 2000和Lipofectamine 3000试剂相比,Transfectamine 5000转染试剂可提供更高的GFP转染效率。
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产品组分
内容 型号
规格 储存温度
Transfectamine 5000转染试剂
60020-01
500ul
-20°C Transfectamine 5000转染试剂
60020-02
1ml
-20°C Transfectamine 5000转染试剂
60020-03
5ml
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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SINE Insertion in the Intron of Pig GHR May Decrease Its Expression by Acting as a Repressor
Authors: Chen Cai, and Zheng, Yao and Wang Mengli, and Murani, Eduard and D'Alessandro, Enrico and Moawad, Ali Shoaib and Wang, Xiaoyan and Wimmers, Klaus and Song, Chengyi
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Journal: Biophysical Journal (2020): 564a--565aLipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells
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Journal: Immunol Cell Biol (2019): 92-96Comparison between Lipofectamine RNAiMAX and GenMute transfection agents in two cellular models of human hepatoma
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Journal: Eur J Histochem (2019): ersion="1.0" encoding="UTF-8" ?>60200.enlEndNCorrection to: Nematollahi et al., Ternary complex of plasmid DNA with NLS-Mu-Mu protein and cationic niosome for biocompatible and efficient gene delivery: a comparative study with protamine and lipofectamine
Authors: name="60200.enl" path="C:\Users\aatbi\Dropbox (AAT Bioquest)\Website Working Files\Product References\60200.enl">60200.enlEndNote4417Correction to: Nematollahi et al., Ternary complex of plasmid DNA with NLS-Mu-Mu protein and cationic niosome for biocompatible , undefined and efficient gene delivery: a comparative study with protamine , undefined and lipofectamineArtif Cells Nanomed BiotechnolArtif Cells Nanomed Biotechnol19924682017/12/062018Dec2169-141X (Electronic) 2169-1401 (Linking)29205060eng Published Erratum Engl, undefined and Artif Cells Nanomed Biotechnol. 2018 Dec;46(8):1992. doi: 10.1080/21691401.2017.1405231. Epub 2017 Dec 5.https://www.ncbi.nlm.nih.gov/pubmed/2920506010.1080/21691401.2017.1405231</electronic-reso, undefined
Journal: Artif Cells Nanomed Biotechnol (2018): 1992Ternary complex of plasmid DNA with NLS-Mu-Mu protein and cationic niosome for biocompatible and efficient gene delivery: a comparative study with protamine and lipofectamine
Authors: Nematollahi, M. H., Torkzadeh-Mahanai, M., Pardakhty, A., Ebrahimi Meim and , H. A., Asadikaram, G.
Journal: Artif Cells Nanomed Biotechnol (2018): 1781-1791The vector-related influences of autophagic microRNA delivery by Lipofectamine 2000 and polyethylenimine 25K on mouse embryonic fibroblast cells
Authors: Lin, C. W., Jan, M. S., Kuo, J. S.
Journal: Eur J Pharm Sci (2017): 11-21The Utilization of RNA Silencing Technology to Mitigate the Voriconazole Resistance of Aspergillus Flavus; Lipofectamine-Based Delivery
Authors: Nami, S., Baradaran, B., Mansoori, B., Kordbacheh, P., Rezaie, S., Falahati, M., Mohamed Khosroshahi, L., Safara, M., Zaini, F.
Journal: Adv Pharm Bull (2017): 53-59The intracellular trafficking mechanism of Lipofectamine-based transfection reagents and its implication for gene delivery
Authors: Cardarelli, F., Digiacomo, L., Marchini, C., Amici, A., Salomone, F., Fiume, G., Rossetta, A., Gratton, E., Pozzi, D., Caracciolo, G.
Journal: Sci Rep (2016): 25879
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