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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C20H27N10O17P3 (free acid)
Molecular Weight: 772.41 g/mol (free acid)
Exact Mass: 772.08 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λ 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Synthesis of mRNA with a non-functional cap analog (ApppG) to estimate the level of cap-independent translation.[1]
Investigation of stress related (Near UV and oxidation) product formation in bacteria[2-4]关键词:- AP3G(A帽)溶液
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产品组分
内容
型号
规格 储存温度
AP3G(A帽)溶液
NU941-01 10ul(100mM)
-20°C AP3G(A帽)溶液
NU941-02 50ul(100mM)
-20°C 操作手册
1 1 常温
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Nowakowska et al. (2014) Cap analogs containing 6-thioguanosine--reagents for the synthesis of mRNAs selectively photo-crosslinkable with cap-binding biomolecules. Org. Biomol. Chem. 12 (27):4841.
[2] Kramer et al. (1988) Near-UV stress in Salmonella typhimurium: 4-thiouridine in tRNA, ppGpp, and ApppGpp as components of an adaptive response. J. Bacteriol. 170 (5):2344.
[3] Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.
[4] VanBogelen et al. (1987) Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli. J. Bacteriol. 169 (1):26.The Cap-Independent Translation of Survivin 5'UTR and HIV-1 IRES Sequences Is Inhibited by Oxidative Stress Produced by H. pylori Gamma-Glutamyl Transpeptidase Activity.
Mariaignacia Rubilar, Nicolás Carrasco-Véliz, ..., Manuel Valenzuela-Valderrama
Biomolecules | 2026 Jan 28 | 41594703
Splicing modulators elicit global translational repression by condensate-prone proteins translated from introns
Shrestha Jagat Krishna Chhipi, Schneider-Poetsch Tilman, ..., Yoshida Minoru
bioRxiv | 2020 Nov 23
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