- 产品描述
- 产品组分
- 文件资源
- 营销中心
- 注意事项
- FAQ
- 文献追踪
-
For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C20H28N10O20P4 (free acid)
Molecular Weight: 852.39 g/mol (free acid)
Exact Mass: 852.04 g/mol (free acid)
CAS#: 10527-46-5
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Formation by T4 RNA ligase[1]
Formation by Pfu DNA ligase[2]
Acceptor substrate for T4 RNA ligase[3] -
产品组分
内容
型号
规格 储存温度
AP4G
NU503-01 50ul(10mM)
-20°C AP4G
NU503-02 250ul(10mM)
-20°C 操作手册
1 1 常温
-
注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。 -
FAQ
-
Selected References:
[1] Sillero et al. (2006) Synthesis of bisphosphonate derivatives of ATP by T4 RNA ligase. FEBS Lett. 580 (24):5723.
[2] Günther et al. (2002) Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates. Extremophiles. 6 (1):45.
[3] Atencia et al. (2000) Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction. Eur. J. Biochem. 267 (6):1707.
Winward et al. (2010) Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? Int. J. Biochem. Cell Biol. 42 (7):1174.
Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster. Int. J. Biochem. Cell Biol. 39 (5):943.
Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.
Ortiz et al. (1993) Specific synthesis of adenosine (5')tetraphospho (5')nucleoside and adenosine- (5')oligophospho (5')adenosine (n-greaterthan- 4) catalyzed by firefly luciverase. Eur. J. Biochem. 212 (1):263.
Palfi et al. (1991) Alterations in the accumulation of adenylylated nucleotides in heavy-metal-ion-stressed and heat-stressed Synechococcus sp strain pcc-6301, a cyanobacterium, in light and dark. Biochem. J. 276:487.
Brevet et al. (1991) Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces-cerevisiae. J. Bacteriol. 173 (17):5275.
Sillero et al. (1991) Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase. Eur. J. Biochem. 202 (2):507.
Avila et al. (1991) A paradoxical increase of a metabolite upon increased expression of its catabolic enzyme - the case of diadenosine tetraphosphate (AP4A) and AP4A phosphorylase-I in Saccharomyces-cerevisiae. J. Bacteriol. 173 (24):7875.
Barnes et al. (1984) Measurement of AP4A and AP4G during the cell-cycle of Physarumpolycephalum. H-S Z. Physiol. Chem. 365 (6):608.
Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.
Garrison et al. (1984) Assay of adenosine 5'-P1-tetraphospho-P4-5'''-adenosine and adenosine 5'-P1-tetraphospho-P4-5'''-guanosine in Physarum-polycephalum and other eukaryotes - an isocratic high-pressure liquid-chromatography method. Biochem. J. 217 (3):805.Molecular insight into 5' RNA capping with Np n Ns by bacterial RNA polymerase.
Valentina M Serianni, Jana Škerlová, ..., Hana Cahova
Nature Chemical Biology | 2026 Jan 09 | 41513851
Molecular basis for non-canonical transcription initiation from Np 4 A alarmones
Wenqian Duan, Abhishek Kaushik, ..., Alexander Serganov
Nature Chemical Biology | 2025 Oct 15 | 41094128
ApaH decaps Np 4 N-capped RNAs in two alternative orientations.
Ashok Nuthanakanti, Megan Korn, ..., Alexander Serganov
Nature Chemical Biology | 2025 Aug 11 | 40789943
Purification and functional characterization of diadenosine 5',5‴-P(1),P(4)-tetraphosphate phosphorylases from Mycobacterium smegmatis and Mycobacterium avium.
Naoko Honda, Hyun Kim, ..., Shigetarou Mori
Protein expression and purification | 2016 Apr 05 | 25940844
Characterisation of a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster.
Lucinda Winward, William G F Whitfield, ..., Stephen T Safrany
The international journal of biochemistry … | 2007 Aug 24 | 17344088
在线留言
如果您对我们的产品感兴趣,请留下您的信息,我们将尽快与您联系,谢谢!
艾维缔官网
艾德官网
B站IVDSHOW
抖音军哥聊表观
视频号艾维缔
小红书艾维缔
快手表观盒子
表观遗传学
联系我们

