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AP4G

AP4G,AppppG,P1-(5'-Adenosyl) P4-(5'-guanosyl) tetraphosphate, Triethylammonium salt,P1-(5'-腺苷基) P4-(5'-鸟苷基) 四磷酸三乙铵盐。更多视频请关注视频号【艾维缔】。哔哩哔哩【IVDSHOW】。抖音【军哥聊表观】。


关键词

AP4G


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目录号

NU503

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  • For general laboratory use.

    Please centrifuge briefly before opening (volume ≤2 ml).

    Shipping: shipped on gel packs

    Storage Conditions: store at -20 °C
    Short term exposure (up to 1 week cumulative) to ambient temperature possible.

    Shelf Life: 12 months after date of delivery

    Molecular Formula: C20H28N10O20P4 (free acid)

    Molecular Weight: 852.39 g/mol (free acid)

    Exact Mass: 852.04 g/mol (free acid)

    CAS#: 10527-46-5

    Purity: ≥ 95 % (HPLC)

    Form: solution in water

    Concentration: 10 mM - 11 mM

    pH: 7.5 ±0.5

    Spectroscopic Properties: λmax 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

    Applications:
    Formation by T4 RNA ligase[1]
    Formation by Pfu DNA ligase[2]
    Acceptor substrate for T4 RNA ligase[3]

  • 产品组分

    内容

    型号

    规格

    储存温度

    AP4G

    NU503-01

    50ul(10mM)

    -20°C

    AP4G

    NU503-02

    250ul(10mM)

    -20°C

    操作手册

    1 1

    常温

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    NU503-AP4G

    操作手册

    NU503-AP4G相关单页

    宣传单页

     

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  • 注意事项

    保存建议 厂家推荐蓝冰运输。当您收到产品后,按照说明书建议保存于-20°C。

     

  • FAQ

     

  • Selected References:
    [1] Sillero et al. (2006) Synthesis of bisphosphonate derivatives of ATP by T4 RNA ligase. FEBS Lett. 580 (24):5723.
    [2] Günther et al. (2002) Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates. Extremophiles. 6 (1):45.
    [3] Atencia et al. (2000) Several dinucleoside polyphosphates are acceptor substrates in the T4 RNA ligase catalyzed reaction. Eur. J. Biochem. 267 (6):1707.
    Winward et al. (2010) Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? Int. J. Biochem. Cell Biol. 42 (7):1174.
    Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogasterInt. J. Biochem. Cell Biol. 39 (5):943.
    Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.
    Ortiz et al. (1993) Specific synthesis of adenosine (5')tetraphospho (5')nucleoside and adenosine- (5')oligophospho (5')adenosine (n-greaterthan- 4) catalyzed by firefly luciverase. Eur. J. Biochem. 212 (1):263.
    Palfi et al. (1991) Alterations in the accumulation of adenylylated nucleotides in heavy-metal-ion-stressed and heat-stressed Synechococcus sp strain pcc-6301, a cyanobacterium, in light and dark. Biochem. J. 276:487.
    Brevet et al. (1991) Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces-cerevisiae. J. Bacteriol. 173 (17):5275.
    Sillero et al. (1991) Synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase. Eur. J. Biochem. 202 (2):507.
    Avila et al. (1991) A paradoxical increase of a metabolite upon increased expression of its catabolic enzyme - the case of diadenosine tetraphosphate (AP4A) and AP4A phosphorylase-I in Saccharomyces-cerevisiae. J. Bacteriol. 173 (24):7875.
    Barnes et al. (1984) Measurement of AP4A and AP4G during the cell-cycle of Physarumpolycephalum. H-S Z. Physiol. Chem. 365 (6):608.
    Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.
    Garrison et al. (1984) Assay of adenosine 5'-P1-tetraphospho-P4-5'''-adenosine and adenosine 5'-P1-tetraphospho-P4-5'''-guanosine in Physarum-polycephalum and other eukaryotes - an isocratic high-pressure liquid-chromatography method. Biochem. J. 217 (3):805.

    Molecular insight into 5' RNA capping with Np n Ns by bacterial RNA polymerase.

    Valentina M Serianni, Jana Škerlová, ..., Hana Cahova

    Nature Chemical Biology  |  2026 Jan 09  |  41513851 

    Molecular basis for non-canonical transcription initiation from Np 4 A alarmones

    Wenqian Duan, Abhishek Kaushik, ..., Alexander Serganov

    Nature Chemical Biology  |  2025 Oct 15  |  41094128

    ApaH decaps Np 4 N-capped RNAs in two alternative orientations.

    Ashok Nuthanakanti, Megan Korn, ..., Alexander Serganov

    Nature Chemical Biology  |  2025 Aug 11  |  40789943

    Purification and functional characterization of diadenosine 5',5‴-P(1),P(4)-tetraphosphate phosphorylases from Mycobacterium smegmatis and Mycobacterium avium.

    Naoko Honda, Hyun Kim, ..., Shigetarou Mori

    Protein expression and purification  |  2016 Apr 05  |  25940844

    Characterisation of a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster.

    Lucinda Winward, William G F Whitfield, ..., Stephen T Safrany

    The international journal of biochemistry …  |  2007 Aug 24  |  17344088

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