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For general laboratory use.
Please centrifuge briefly before opening (volume ≤2 ml).Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.Shelf Life: 12 months after date of delivery
Molecular Formula: C20H29N10O23P5 (free acid)
Molecular Weight: 932.37 g/mol (free acid)
Exact Mass: 932.01 g/mol (free acid)
CAS#: 56983-24-5
Purity: ≥ 95 % (HPLC)
Form: solution in water
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Substrate replacement at UMP-CMP kinase[1]
Crystal structure of complex with GMP kinase[2]
Vasoconstriction via activation of P2X-receptors[3]
Activation of P2X receptor ion channels[4]Specific Ligands:
Binding to purinoreceptors P2Y1 and P2X[5]
Binding to purinoreceptors P2X3 and rP2X1[6]
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产品组分
内容
型号
规格 储存温度
AP5G
NU504-01 50ul(10mM)
-20°C AP5G
NU504-02 250ul(10mM)
-20°C 操作手册
1 1 常温
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营销中心
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注意事项
保存建议 厂家推荐蓝冰运输。当您收到产品后，按照说明书建议保存于-20°C。 -
FAQ
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Selected References:
[1] Topalis et al. (2007) Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives -- a screening based on affinity for the UMP-CMP binding site. FEBS J. 274 (14):3704.
[2] Hible et al. (2006) Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G. Biochimie 88:1157.
[3] van der Giet et al. (2001) The critical role of adenosine and guanosine in the affinity of dinucleoside polyphosphates to P (2X)-receptors in the isolated perfused rat kidney. Br. J. Pharmacol. 132 (2):467.
[4] Lewis et al. (2000)Effects of diadenosine polyphosphates (Ap (n)As) and adenosine polyphospho guanosines (Ap (n)Gs) on rat mesenteric artery P2X receptor ion channels.Br. J. Pharmacol. 129 (1):124.
[5] van der Giet et al. (2002) Effects of dinucleoside polyphosphates on regulation of coronary vascular tone. Eur. J. Pharmacol. 448 (2-3):207.
[6] Cinkilic et al. (2001) Selective agonism of group I P2X receptors by dinucleotides dependent on a single adenine moiety. J. Pharmacol. Exp. Ther. 299 (1):131.
Winward et al. (2010) Oxidation of the diphosphoinositol polyphosphate phosphohydrolase-like Nudix hydrolase Aps from Drosophila melanogaster induces thermolability--A possible regulatory switch? Int. J. Biochem. Cell Biol. 42 (7):1174.
Safrany et al. (2007) Characterisation of a bis (5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster. Int. J. Biochem. Cell Biol. 39 (5):943.
Cherfils et al. (2006) Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G. Biochimie 88 (9):1157.
Gross et al. (2006) Nucleotide-binding domains of Cystic Fibrosis Transmembrane Conductance Regulator, an ABC Transporter, Catalyze Adenylate Kinase Activity but not ATP Hydrolysis. J. Biol. Chem. 281 (7):4058.
Leslie et al. (2002) Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases. BMC Biochemistry 3:20.
Prinz et al. (1999) Binding of nucleotides to guanylate kinase, p21 (ras), and nucleoside-diphosphate kinase studied by nano-electrospray mass spectrometry. J. Biol. Chem. 274 (50):35337.
Ortiz et al. (1993) Specific synthesis of adenosine (5')tetraphospho (5')nucleoside and adenosine- (5')oligophospho (5')adenosine (n > 4) catalyzed by firefly luciverase. Eur. J. Biochem. 212 (1):263.
Feldhaus et al. (1975) Synthetic Inhibitors of Adenylate Kinases in the Assays for ATPases and Phosphokinases. Eur. J. Biochem. 57:197.Guanylate Kinase 1 Deficiency: A Novel and Potentially Treatable Mitochondrial DNA Depletion/Deletions Disease.
Agustin Hidalgo-Gutierrez, Jonathan Shintaku, ..., Michio Hirano
Annals of Neurology | 2024 Sep 04 | 39230499
Purification and functional characterization of diadenosine 5',5‴-P(1),P(4)-tetraphosphate phosphorylases from Mycobacterium smegmatis and Mycobacterium avium.
Naoko Honda, Hyun Kim, ..., Shigetarou Mori
Protein expression and purification | 2016 Apr 05 | 25940844
Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives -- a screening based on affinity for the UMP-CMP binding site.
Dimitri Topalis, Hiroki Kumamoto, ..., Dominique Deville-Bonne
The Febs Journal | 2007 Aug 30 | 17608725
Characterisation of a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster.
Lucinda Winward, William G F Whitfield, ..., Stephen T Safrany
The international journal of biochemistry … | 2007 Aug 24 | 17344088
Crystal structures of GMP kinase in complex with ganciclovir monophosphate and Ap5G.
G Hible, P Daalova, ..., J Cherfils
Biochimie | 2007 Jan 23 | 16690197
Nucleotide-binding Domains of Cystic Fibrosis Transmembrane Conductance Regulator, an ABC Transporter, Catalyze Adenylate Kinase Activity but Not ATP Hydrolysis
Christian H. Gross, Norzehan Abdul-Manan, ..., Jonathan Moore
Journal of Biological Chemistry | 2006 Feb 01
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